| Fiddlestiks Food and Spirits Company | Retail Trade | $334,603 | $336,555 | 91 | $22.7M |  Research |
| Ehrhardt Hospitality, LLC | Accommodation and Food Services | $326,400 | $328,510 | 58 | $18.1M | Research |
| Wright Furniture, INC | Retail Trade | $301,900 | $303,903 | 29 | $5.3M | Research |
| Hannibal Sandys INC | Other Services (except Public Administration) | $253,475 | $254,968 | 71 | $13.9M | Research |
| Young Mens Christian Association | Health Care and Social Assistance | $248,615 | $198,218 | 95 | N/A | Research |
| Alan M. Stoll, D.d.s., P.c. | Health Care and Social Assistance | $239,822 | $72,802 | 93 | $21.5M | Research |
| Poage INC | Retail Trade | $233,400 | $234,999 | 28 | $7.1M | Research |
| Riverside Dermatology & Spa LLC | Health Care and Social Assistance | $233,082 | $234,396 | 27 | $8.5M | Research |
| Holy Family Catholic Church, Hannibal | Other Services (except Public Administration) | $199,200 | $200,323 | 40 | N/A | Research |
| Poage Chrysler Dodge Jeep INC | Retail Trade | $197,500 | $198,847 | 20 | $5.0M | Research |
| Marion County Services Inc. | Health Care and Social Assistance | $196,482 | $197,188 | 32 | N/A | Research |
| Wasinger Parham, L.c. | Professional, Scientific, and Technical Services | $191,250 | $192,350 | 12 | <$5M | Research |
| Wasinger Parham, L.c. | Professional, Scientific, and Technical Services | $187,599 | $188,086 | 12 | <$5M | Research |
| Big River Oil Company | Wholesale Trade | $186,122 | $187,063 | 22 | $5.7M | Research |
| Golden Eacgle Spotting Company | Wholesale Trade | $178,782 | $179,781 | 16 | <$5M | Research |
| Big River Oil Company | Mining, Quarrying, and Oil and Gas Extraction | $176,494 | $177,661 | 18 | <$5M | Research |
| Park Place Sign Systems, Inc. | Administrative and Support and Waste Management | $170,000 | $170,978 | 23 | <$5M | Research |
| Lomax Trucking Service INC | Transportation and Warehousing | $161,500 | $163,057 | 17 | <$5M | Research |
| Mtbc, LLC | Accommodation and Food Services | $151,400 | $152,877 | 39 | $6.9M | Research |
| Lomax Trucking Service INC | Transportation and Warehousing | $149,965 | $150,857 | 17 | <$5M | Research |
| Thompson's Kitchen of Hannibal | Accommodation and Food Services | $149,754 | $150,332 | 20 | <$5M | Research |
| Calvary Baptist Church | Other Services (except Public Administration) | $141,513 | $142,605 | 21 | N/A | Research |
| Mark Twain Dinette Inc. | Accommodation and Food Services | $140,392 | $140,885 | 16 | <$5M | Research |
| Cassano Pizza King of Hannibal INC | Accommodation and Food Services | $138,900 | $139,838 | 37 | $6.6M | Research |
| Northeast Independent Living Services | Health Care and Social Assistance | $134,000 | $135,180 | 26 | N/A | Research |