| Thompson's Kitchen of Hannibal | Accommodation and Food Services | $132,476 | $133,613 | 31 | $5.5M |  Research |
| The Golden Ruler Inc. | Retail Trade | $132,156 | $133,078 | 15 | <$5M | Research |
| Hrw,inc | Unknown | $131,600 | $133,190 | 24 | N/A | Research |
| Legacy Hospitality Group LLC | Accommodation and Food Services | $130,333 | $130,983 | 17 | $5.3M | Research |
| Howell and Hart Enterprises LLC | Accommodation and Food Services | $128,064 | $129,050 | 15 | <$5M | Research |
| M Twain Cave Inc. | Arts, Entertainment, and Recreation | $127,000 | $127,720 | 40 | $5.6M | Research |
| Sbd Enterprise INC | Other Services (except Public Administration) | $126,320 | $127,401 | 20 | <$5M | Research |
| St. John's Evangelical Lutheran Church and School | Other Services (except Public Administration) | $126,100 | $126,946 | 28 | N/A | Research |
| Sdi of Hannibal LLC | Accommodation and Food Services | $125,300 | $126,344 | 57 | $10.1M | Research |
| James O'donnell Funeral Home, INC | Other Services (except Public Administration) | $123,200 | $124,125 | 21 | <$5M | Research |
| Skyview Construction & Crane, LLC | Construction | $117,500 | $118,783 | 19 | $5.9M | Research |
| Eew LLC | Accommodation and Food Services | $117,404 | $118,001 | 17 | $5.3M | Research |
| The Golden Ruler INC | Retail Trade | $114,102 | $115,170 | 17 | <$5M | Research |
| Capital Hospitality Group LLC | Accommodation and Food Services | $113,053 | $113,614 | 57 | $17.8M | Research |
| Legacy Hospitality Group, LLC | Accommodation and Food Services | $112,800 | $113,489 | 18 | $5.6M | Research |
| Oak Hospitality LLC | Accommodation and Food Services | $112,000 | $112,604 | 23 | $7.2M | Research |
| Shaw Restaurant, INC | Accommodation and Food Services | $106,715 | $107,216 | 17 | <$5M | Research |
| Abuse Victims Education Network Unified to Ensure Safety for | Health Care and Social Assistance | $106,698 | $107,128 | 18 | <$5M | Research |
| Swb Hospitality LLC | Accommodation and Food Services | $103,235 | $0 | 23 | $7.2M | Research |
| Immanuel Baptist Church | Other Services (except Public Administration) | $101,400 | $53,554 | 18 | N/A | Research |
| Logue's Restaurant, LLC | Accommodation and Food Services | $100,000 | $100,564 | 27 | <$5M | Research |
| Java Jive, LLC | Accommodation and Food Services | $99,512 | $99,894 | 22 | <$5M | Research |
| Big River Pest Control | Administrative and Support and Waste Management | $96,954 | $97,595 | 13 | <$5M | Research |
| Marion County Services, INC | Health Care and Social Assistance | $96,800 | $97,508 | 39 | N/A | Research |
| Szc Hotels LLC | Accommodation and Food Services | $92,959 | $93,390 | 19 | $5.9M | Research |