| Preferred Family Healthcare Incorporated | Health Care and Social Assistance | $10,000,000 | $10,032,222 | 500 | $89.0M |  Research |
| Northeast Missouri Health Council | Health Care and Social Assistance | $1,797,592 | $1,807,540 | 156 | N/A | Research |
| Chariton Valley Association, INC | Health Care and Social Assistance | $879,170 | $884,036 | 180 | N/A | Research |
| Kirksville Motor Company | Retail Trade | $554,900 | $561,389 | 52 | $13.1M | Research |
| Bowling Green C and R INC | Retail Trade | $319,815 | $321,841 | 115 | $28.7M | Research |
| Northern Missouri Managements | Retail Trade | $297,827 | $299,688 | 25 | $6.2M | Research |
| Cleaver Dermatology, PC | Health Care and Social Assistance | $254,985 | $256,606 | 34 | $10.7M | Research |
| Cleaver Dermatology PC | Health Care and Social Assistance | $254,985 | $256,452 | 25 | $7.8M | Research |
| Macon Consumers INC | Retail Trade | $209,496 | $210,892 | 63 | $15.7M | Research |
| Kirksville Restaurant Investors | Accommodation and Food Services | $206,224 | $207,834 | 90 | $16.0M | Research |
| Psr Construction Inc. | Construction | $205,705 | $206,962 | 13 | $14.8M | Research |
| S.c.a.r.e.d., Llc, Inc. | Retail Trade | $205,208 | $206,507 | 69 | $17.2M | Research |
| Psr Construction, INC | Construction | $200,844 | $201,967 | 11 | $12.5M | Research |
| Kirksville Dental Group LLP | Health Care and Social Assistance | $195,612 | $197,268 | 22 | $5.1M | Research |
| Lloyd Distributing Co., Inc. | Wholesale Trade | $189,647 | $190,713 | 28 | $7.2M | Research |
| Pan Cit, INC | Accommodation and Food Services | $170,265 | $171,016 | 33 | $5.9M | Research |
| B & H Petroleum Corporation | Other Services (except Public Administration) | $166,700 | $167,618 | 19 | <$5M | Research |
| Sparks Constructors INC | Construction | $161,226 | $159,752 | 17 | <$5M | Research |
| Dki Inc. | Accommodation and Food Services | $158,379 | $159,728 | 17 | <$5M | Research |
| Lewellen's Professional Cleaning System Inc. | Administrative and Support and Waste Management | $153,608 | $154,791 | 14 | <$5M | Research |
| Lewellen's Professional Cleaning System Inc. | Administrative and Support and Waste Management | $153,608 | $154,521 | 14 | <$5M | Research |
| Hospice 2000 Inc. | Health Care and Social Assistance | $153,292 | $154,144 | 15 | N/A | Research |
| Weber Bus Inc. | Transportation and Warehousing | $148,389 | $148,971 | 54 | $20.8M | Research |
| Grgurich and Grgurich Trucking LLC | Transportation and Warehousing | $148,059 | $149,170 | 22 | $5.9M | Research |
| Heartland Surgical Specialists, L.l.p. | Health Care and Social Assistance | $137,900 | $138,659 | 10 | <$5M | Research |