| Three Rivers College | Educational Services | $2,349,900 | $2,380,057 | 302 | N/A |  Research |
| Ozark Border Electric Cooperative | Utilities | $1,800,907 | $1,810,755 | 95 | N/A | Research |
| M&a Electric Power Cooperative | Utilities | $1,222,700 | $1,236,693 | 46 | N/A | Research |
| Hand Cut Steaks of Poplar Bluff, Inc. | Accommodation and Food Services | $972,700 | $982,184 | 264 | $46.9M | Research |
| Starting USA Corporation | Manufacturing | $896,307 | $905,245 | 86 | $22.5M | Research |
| Robertson Contractors, Inc. | Construction | $890,500 | $895,575 | 62 | $70.6M | Research |
| Las Margaritas INC | Accommodation and Food Services | $886,900 | $893,897 | 179 | $31.8M | Research |
| Home Care Equipment Co, Inc. | Health Care and Social Assistance | $829,200 | $837,239 | 91 | $15.6M | Research |
| Health Partners Management Group Inc. | Professional, Scientific, and Technical Services | $812,700 | $817,892 | 141 | $44.9M | Research |
| Kajacs Contractors, INC | Construction | $799,100 | $806,447 | 138 | $42.8M | Research |
| Baldwin Ford Lincoln Toyota | Retail Trade | $724,600 | $729,508 | 114 | $28.7M | Research |
| Hillis Auto Sales INC | Retail Trade | $723,200 | $630,920 | 55 | $13.9M | Research |
| Gregory Logistics Inc. | Transportation and Warehousing | $715,200 | $719,610 | 57 | $11.5M | Research |
| Huffman Construction LLC | Construction | $677,727 | $681,153 | 36 | $41.0M | Research |
| Huffman Construction, LLC | Construction | $677,700 | $685,512 | 22 | $6.8M | Research |
| Ozark Physical Medicine Center for Operations, LLC | Health Care and Social Assistance | $643,158 | $0 | 99 | $17.0M | Research |
| Schaller Hardwood Lumber Company | Wholesale Trade | $636,700 | $643,226 | 50 | $15.3M | Research |
| Palladian Senior Care of Poplar Bluff LLC | Health Care and Social Assistance | $562,500 | $566,609 | 158 | $26.9M | Research |
| Ozark Physical Medicine Center for Operations, LLC | Health Care and Social Assistance | $550,400 | $554,577 | 87 | $14.9M | Research |
| Pdq Imaging Services LLC | Health Care and Social Assistance | $525,400 | $529,647 | 56 | $13.0M | Research |
| S. H. Smith & Company Inc. | Professional, Scientific, and Technical Services | $519,900 | $523,900 | 39 | $7.9M | Research |
| Hefner Furniture & Appliance INC | Retail Trade | $515,900 | $518,809 | 71 | $12.9M | Research |
| Baldwin Chevrolet Cadillac INC | Retail Trade | $466,600 | $469,672 | 82 | $20.7M | Research |
| Cedargate Healthcare Group, LLC | Health Care and Social Assistance | $441,940 | $447,752 | 79 | $13.4M | Research |
| Independent Living Center of Semo | Health Care and Social Assistance | $432,400 | $435,511 | 50 | N/A | Research |