| Butler County Ems LLC | Health Care and Social Assistance | $410,912 | $412,702 | 53 | $12.3M |  Research |
| The Dermalology Office, Inc. | Health Care and Social Assistance | $410,100 | $412,918 | 43 | $13.5M | Research |
| H. Blackwell Oldsmobile-Cad.,i | Retail Trade | $397,100 | $399,714 | 80 | $20.2M | Research |
| Mclane Livestock Transport | Transportation and Warehousing | $393,500 | $397,413 | 41 | $10.9M | Research |
| Dermatology Office PC | Health Care and Social Assistance | $381,200 | $382,905 | 43 | $13.5M | Research |
| Buffington Brothers Heating & Air Conditioning INC | Construction | $365,900 | $368,197 | 29 | $9.2M | Research |
| Buffington Bros Heating and Cooling | Construction | $365,900 | $368,116 | 32 | $10.1M | Research |
| R.l. Persons Construction Inc. | Construction | $358,700 | $361,570 | 24 | $7.4M | Research |
| Coffman and Company PC | Professional, Scientific, and Technical Services | $356,400 | $359,519 | 31 | $9.9M | Research |
| Dental Arts Group LLC | Health Care and Social Assistance | $356,300 | $358,477 | 31 | $7.2M | Research |
| Dental Arts Group, LLC | Health Care and Social Assistance | $355,700 | $358,341 | 30 | $7.0M | Research |
| Butler County Ems, LLC | Health Care and Social Assistance | $352,500 | $356,877 | 57 | $13.2M | Research |
| N and R of Poplar Bluff, INC | Health Care and Social Assistance | $349,600 | $353,708 | 79 | $13.4M | Research |
| Robertson INC Bridge & Grading Division | Construction | $347,000 | $349,605 | 30 | $9.3M | Research |
| Kraft, Miles, and Tatum, LLC | Professional, Scientific, and Technical Services | $312,500 | $314,897 | 25 | $8.0M | Research |
| Ridge Maud Drug Corporation | Retail Trade | $308,500 | $311,086 | 47 | $8.2M | Research |
| Internal Medicine Associates of Southeast Missouri, LLC | Health Care and Social Assistance | $301,300 | $303,275 | 14 | <$5M | Research |
| Joe's Bridge & Grading, Inc. | Construction | $296,300 | $298,008 | 21 | $6.5M | Research |
| Butler County Publishing, LLC | Information | $295,800 | $298,093 | 35 | $5.9M | Research |
| Poplar Bluff Realty, INC | Real Estate and Rental and Leasing | $288,300 | $291,055 | 30 | <$5M | Research |
| Butler County Publishing LLC | Information | $279,700 | $281,118 | 37 | $6.2M | Research |
| Boycom Cablevision, Inc. | Information | $279,000 | $280,828 | 18 | <$5M | Research |
| Pmb, Inc. | Manufacturing | $267,500 | $269,559 | 30 | $7.9M | Research |
| Pmb Inc. | Manufacturing | $265,416 | $266,827 | 29 | $28.8M | Research |
| Heartland Group Insurance Agency, Inc. | Finance and Insurance | $257,700 | $259,525 | 24 | $12.3M | Research |