| Brewer Science INC | Manufacturing | $5,581,900 | $5,647,332 | 365 | $95.6M |  Research |
| Weinbaum Masada LLC | Accommodation and Food Services | $1,323,300 | $1,341,312 | 267 | $47.4M | Research |
| Henry J. Antolak Dmd, PC | Health Care and Social Assistance | $986,000 | $995,644 | 96 | $22.2M | Research |
| Grellner Sales & Service, INC | Wholesale Trade | $714,700 | $720,001 | 80 | $20.6M | Research |
| South Central Missouri Community Health Center | Health Care and Social Assistance | $659,137 | $665,765 | 49 | N/A | Research |
| Mo-Sci Corporation | Manufacturing | $609,757 | $614,318 | 43 | $42.6M | Research |
| Robertson & Penn, Inc. | Other Services (except Public Administration) | $535,073 | $538,164 | 70 | $13.4M | Research |
| Robertson & Penn Inc. | Other Services (except Public Administration) | $531,676 | $534,777 | 48 | $9.2M | Research |
| Choices for People Center for Citizens With Disabilities, in | Health Care and Social Assistance | $500,000 | $504,083 | 110 | N/A | Research |
| West Family Motors LLC | Retail Trade | $484,659 | $487,904 | 37 | $9.3M | Research |
| Silverstone Snf LLC | Health Care and Social Assistance | $472,232 | $477,097 | 89 | $15.1M | Research |
| Mi Serenity LLC | Health Care and Social Assistance | $443,881 | $447,654 | 223 | $38.2M | Research |
| Fairground Auto Plaza, Inc. | Retail Trade | $437,800 | $440,779 | 45 | $11.3M | Research |
| Future Care Inc. | Health Care and Social Assistance | $365,935 | $367,529 | 55 | $8.9M | Research |
| Rolla Grand LLC | Health Care and Social Assistance | $363,141 | $367,111 | 66 | $11.2M | Research |
| Phelps County Restaurant LLC | Accommodation and Food Services | $339,913 | $342,251 | 60 | $10.7M | Research |
| Tlc Home Health Care, LLC | Health Care and Social Assistance | $332,500 | $334,449 | 49 | $8.4M | Research |
| Cm Archer Group, P.c. | Professional, Scientific, and Technical Services | $327,429 | $329,530 | 33 | $10.2M | Research |
| Donald Maggi, Inc. | Construction | $313,535 | $315,503 | 27 | $8.4M | Research |
| Taylor Automotive Group LLC | Retail Trade | $284,759 | $286,871 | 48 | $12.1M | Research |
| Taylor Automotive Group LLC | Retail Trade | $284,759 | $287,084 | 32 | $8.1M | Research |
| Sakelaris Ford Lincoln of Rolla, Inc. | Retail Trade | $278,910 | $281,629 | 25 | $6.3M | Research |
| Stevens Distributors, Incorporated | Transportation and Warehousing | $275,737 | $277,334 | 25 | $5.0M | Research |
| Investment Realty, Inc. | Real Estate and Rental and Leasing | $259,004 | $260,982 | 40 | $5.8M | Research |
| Subway of Rolla INC | Accommodation and Food Services | $252,213 | $253,558 | 70 | $12.4M | Research |