| Keller's Rental Place, Inc. | Real Estate and Rental and Leasing | $172,328 | $173,492 | 24 | $10.8M |  Research |
| Tater Patch Saloon LLC | Accommodation and Food Services | $170,391 | $171,633 | 31 | $5.5M | Research |
| Cub Creek INC | Health Care and Social Assistance | $154,710 | $156,055 | 12 | <$5M | Research |
| The Dermatology Center LLC | Health Care and Social Assistance | $154,323 | $155,146 | 15 | <$5M | Research |
| Thomas, Birdsong, Mills, Mcbride & Osborne, P.c. | Professional, Scientific, and Technical Services | $150,560 | $151,614 | 13 | <$5M | Research |
| Laci's Haven INC | Other Services (except Public Administration) | $147,566 | $148,312 | 20 | <$5M | Research |
| Pro Mo Contracting Services LLC | Construction | $146,800 | $147,653 | 23 | $8.6M | Research |
| Rolla Farmers Exchange | Retail Trade | $143,538 | $144,722 | 25 | $5.2M | Research |
| Thomas Birdsong Mills Mcbride & Osborne P.c. | Professional, Scientific, and Technical Services | $141,948 | $142,500 | 13 | <$5M | Research |
| Burin, LLC | Accommodation and Food Services | $141,300 | $142,423 | 30 | $5.3M | Research |
| Paragon Business Solutions, Inc. | Professional, Scientific, and Technical Services | $140,495 | $141,693 | 11 | <$5M | Research |
| Absolute Garage Door Repair | Construction | $139,585 | $107,697 | 12 | <$5M | Research |
| Amc Rolla, Inc. | Accommodation and Food Services | $139,400 | $140,531 | 40 | $7.1M | Research |
| Jackson and Son Trucking, INC | Transportation and Warehousing | $133,241 | $134,267 | 17 | <$5M | Research |
| Non Profit | Health Care and Social Assistance | $132,037 | $74,556 | 18 | N/A | Research |
| Promow, Inc. | Accommodation and Food Services | $130,200 | $131,325 | 26 | <$5M | Research |
| Integrity Engineering INC | Professional, Scientific, and Technical Services | $129,975 | $130,516 | 6 | <$5M | Research |
| S.w. Tax Authority, Inc. | Professional, Scientific, and Technical Services | $128,081 | $114,140 | 47 | $15.0M | Research |
| Hi-Tech Collision LLC | Other Services (except Public Administration) | $124,721 | $125,784 | 14 | <$5M | Research |
| Rolla Urgent Care Llc, DBA Rolla Family Clinic | Health Care and Social Assistance | $121,753 | $122,930 | 10 | <$5M | Research |
| Alex Pizza | Accommodation and Food Services | $121,005 | $121,745 | 15 | <$5M | Research |
| Christian Life Center | Other Services (except Public Administration) | $119,617 | $120,275 | 36 | $6.9M | Research |
| Calvin L. Mott D.m.d LLC | Health Care and Social Assistance | $119,098 | $85,763 | 9 | <$5M | Research |
| Mid-Missouri Foot & Ankle Center, Inc. | Health Care and Social Assistance | $118,757 | $119,770 | 15 | <$5M | Research |
| Gabriele Crane Rental Inc. | Real Estate and Rental and Leasing | $117,042 | $117,982 | 9 | <$5M | Research |