| Platte Center West LLC | Agriculture, Forestry, Fishing and Hunting | $1,901,190 | $1,923,265 | 304 | $49.7M |  Research |
| Daniels Produce LLC | Agriculture, Forestry, Fishing and Hunting | $1,308,320 | $1,318,096 | 183 | $18.7M | Research |
| Dewine Mechanical, INC | Construction | $1,268,143 | $1,283,149 | 43 | $49.0M | Research |
| Rvw, Inc. | Manufacturing | $1,254,034 | $1,267,619 | 65 | $17.0M | Research |
| Dna Production, LLC | Agriculture, Forestry, Fishing and Hunting | $1,227,188 | $1,237,959 | 146 | $14.9M | Research |
| Columbus Operations LLC | Health Care and Social Assistance | $1,043,467 | $1,053,177 | 141 | $24.0M | Research |
| Fremarek INC | Wholesale Trade | $1,008,400 | $1,015,571 | 84 | $25.7M | Research |
| Gjw LLC | Agriculture, Forestry, Fishing and Hunting | $877,009 | $885,657 | 270 | $44.2M | Research |
| Gehring Construction & Ready Mix Co INC | Manufacturing | $868,295 | $871,602 | 58 | $57.5M | Research |
| B-D Construction, INC | Construction | $805,509 | $813,161 | 83 | $94.6M | Research |
| B-D Construction, INC | Construction | $805,500 | $811,497 | 83 | $94.6M | Research |
| Walker Foundation, Inc. | Construction | $784,114 | $791,160 | 51 | $11.8M | Research |
| Jackson Services, Inc. | Other Services (except Public Administration) | $740,400 | $746,488 | 76 | $14.5M | Research |
| Pillen Family Farms Transport, LLC | Transportation and Warehousing | $727,770 | $733,147 | 90 | $24.0M | Research |
| Ernst Auto Center, Inc. | Retail Trade | $720,755 | $727,742 | 50 | $12.6M | Research |
| Central Confinement Service, LLC | Construction | $685,620 | $689,105 | 56 | $63.8M | Research |
| Vsl Columbus LLC | Health Care and Social Assistance | $674,942 | $679,742 | 127 | $21.6M | Research |
| Bierman Contracting INC | Construction | $648,915 | $653,661 | 48 | $54.7M | Research |
| Sandy Pine Systems INC | Agriculture, Forestry, Fishing and Hunting | $648,793 | $655,281 | 270 | $44.2M | Research |
| Sandhills Elite Genetics LLC | Agriculture, Forestry, Fishing and Hunting | $626,553 | $653,770 | 270 | $44.2M | Research |
| Pillen Family Farms INC | Agriculture, Forestry, Fishing and Hunting | $623,685 | $628,016 | 49 | $8.0M | Research |
| Columbus Family Practice Associates PC | Health Care and Social Assistance | $602,706 | $606,942 | 52 | $16.3M | Research |
| Columbus Family Practice Associates, P.c. | Health Care and Social Assistance | $602,700 | $607,488 | 55 | $17.3M | Research |
| Pillen Family Farms, LLC | Agriculture, Forestry, Fishing and Hunting | $595,910 | $602,895 | 78 | $12.8M | Research |
| Duo Lift Manufacturing Company, Inc. | Manufacturing | $590,515 | $595,563 | 72 | $18.9M | Research |