| Grosch Irrigation Company | Manufacturing | $901,030 | $907,638 | 89 | $23.3M |  Research |
| Arbor Care Centers- O'neill LLC | Health Care and Social Assistance | $481,300 | $485,752 | 54 | $9.2M | Research |
| Wm Krotter Co | Retail Trade | $347,750 | $350,030 | 50 | $12.6M | Research |
| St. Patrick's Catholic Church | Other Services (except Public Administration) | $293,300 | $265,805 | 52 | N/A | Research |
| John Prouty Construction, Inc. | Construction | $267,240 | $268,953 | 44 | $50.1M | Research |
| John Prouty Construction Inc. | Construction | $262,471 | $286,864 | 31 | $11.6M | Research |
| Building Blocks for Community Enrichment | Health Care and Social Assistance | $220,600 | $221,777 | 18 | N/A | Research |
| Sandhills Investors LLC | Health Care and Social Assistance | $189,673 | $190,405 | 38 | $6.5M | Research |
| Torpins Rodeo Market, INC | Retail Trade | $175,200 | $176,353 | 40 | $10.0M | Research |
| Kersenbrock Dental Care LLC | Health Care and Social Assistance | $171,100 | $172,041 | 13 | <$5M | Research |
| Oneill Family Pharmacy INC | Wholesale Trade | $159,300 | $155,353 | 33 | $8.5M | Research |
| Dennis Baumert Cattle Company L.l.c. | Agriculture, Forestry, Fishing and Hunting | $156,080 | $156,739 | 13 | <$5M | Research |
| Kersenbrock Dental Care LLC | Health Care and Social Assistance | $155,910 | $156,573 | 13 | <$5M | Research |
| Elkhorn Valley Family Medicine PC | Health Care and Social Assistance | $154,483 | $155,372 | 11 | <$5M | Research |
| H H Hall Restaurant of O'neill Inc. | Accommodation and Food Services | $145,900 | $146,719 | 22 | <$5M | Research |
| Adamson Distributing Co. Inc. | Wholesale Trade | $142,500 | $143,866 | 16 | <$5M | Research |
| Opportunity Farms | Agriculture, Forestry, Fishing and Hunting | $139,508 | $139,988 | 16 | <$5M | Research |
| Opportunity Farms | Agriculture, Forestry, Fishing and Hunting | $127,800 | $128,708 | 15 | <$5M | Research |
| O'neill Sl, Inc. | Health Care and Social Assistance | $125,100 | $126,306 | 15 | <$5M | Research |
| North Central Automation Inc. | Wholesale Trade | $124,400 | $125,330 | 10 | <$5M | Research |
| Family Dentistry & Dental Hygiene, P.c. | Health Care and Social Assistance | $123,900 | $124,757 | 13 | <$5M | Research |
| Schmitz Electric INC | Construction | $122,135 | $122,830 | 9 | <$5M | Research |
| Elkhorn Valley Family Medicine | Health Care and Social Assistance | $116,500 | $117,232 | 11 | <$5M | Research |
| Holt County Ford Inc. | Wholesale Trade | $115,900 | $116,708 | 11 | <$5M | Research |
| Prairie Hills Ford | Retail Trade | $110,291 | $110,671 | 11 | <$5M | Research |