| Eastern Niagara Hospital | Health Care and Social Assistance | $5,853,436 | $5,917,423 | 480 | N/A |  Research |
| New Directions Youth and Family Serivces, Inc. | Health Care and Social Assistance | $3,461,305 | $3,505,780 | 415 | N/A | Research |
| New York Beer Project LLC | Accommodation and Food Services | $1,815,744 | $1,836,986 | 86 | $15.3M | Research |
| Candlelight Cabinetry, Inc. | Manufacturing | $1,664,500 | $1,681,327 | 205 | $53.7M | Research |
| Niagara Hospice, Inc. | Health Care and Social Assistance | $1,562,000 | $1,582,113 | 166 | N/A | Research |
| Reid Petroleum Inc. | Wholesale Trade | $1,519,445 | $1,533,266 | 94 | $24.2M | Research |
| 104 Old Niagara Road Operating Company LLC | Health Care and Social Assistance | $1,386,550 | $1,421,483 | 177 | $30.1M | Research |
| New York Beer Project LLC | Manufacturing | $1,296,900 | $1,313,244 | 225 | $79.4M | Research |
| Intertech Digital Entertainment INC | Information | $1,135,143 | $1,142,109 | 214 | $35.9M | Research |
| Intertech Digital Entertainment INC | Information | $1,128,200 | $1,143,995 | 115 | $19.3M | Research |
| Clark Rigging & Rental Corp. | Real Estate and Rental and Leasing | $1,095,410 | $1,102,883 | 57 | $25.8M | Research |
| Clark Rigging & Rental Corp | Real Estate and Rental and Leasing | $1,095,400 | $1,109,295 | 36 | $16.3M | Research |
| Diversified Manufacturing INC | Manufacturing | $1,094,267 | $1,101,552 | 106 | $25.9M | Research |
| Diversified Manufacturing INC | Administrative and Support and Waste Management | $1,093,445 | $1,098,807 | 90 | $28.5M | Research |
| Bison Bag Co. Inc. | Manufacturing | $1,050,312 | $1,056,182 | 95 | $24.9M | Research |
| Custom Laser INC | Manufacturing | $956,867 | $968,009 | 70 | $17.1M | Research |
| Custom Laser, INC | Manufacturing | $956,800 | $964,533 | 71 | $17.4M | Research |
| Maximus 909 Operations, LLC | Health Care and Social Assistance | $949,354 | $961,300 | 93 | $15.8M | Research |
| Relco Systems, Inc. | Transportation and Warehousing | $925,000 | $935,669 | 84 | $12.7M | Research |
| Relco Systems Inc. | Transportation and Warehousing | $900,000 | $911,786 | 76 | $15.3M | Research |
| Heinrich Chevrolet Corp. | Retail Trade | $896,435 | $900,389 | 54 | $13.6M | Research |
| Heinrich Chevrolet Corporation | Retail Trade | $896,400 | $904,013 | 74 | $18.6M | Research |
| Reid Stores Inc. | Retail Trade | $851,600 | $860,956 | 131 | $43.8M | Research |
| National Recovery Solutions, LLC | Administrative and Support and Waste Management | $835,800 | $844,250 | 91 | $17.2M | Research |
| Basil Family Dealerships | Retail Trade | $769,142 | $774,031 | 61 | $15.4M | Research |