| Mount View Assisted Living, Inc. | Health Care and Social Assistance | $467,195 | $472,672 | 83 | $14.1M |  Research |
| Ywca of the Niagara Frontier | Other Services (except Public Administration) | $437,947 | $440,899 | 68 | $13.0M | Research |
| Ywca of the Niagara Frontier, INC | Other Services (except Public Administration) | $437,900 | $442,999 | 95 | $18.1M | Research |
| John R. Freundschuh, Inc. | Wholesale Trade | $437,225 | $439,800 | 27 | $8.3M | Research |
| Altitude Marketing LLC | Finance and Insurance | $422,715 | $426,676 | 214 | $109.9M | Research |
| Altitude Marketing LLC | Administrative and Support and Waste Management | $409,780 | $414,686 | 44 | $8.3M | Research |
| Contracts Unlimited INC | Manufacturing | $400,667 | $402,577 | 36 | $8.8M | Research |
| Great Lakes Asset Solutions, LLC | Administrative and Support and Waste Management | $375,000 | $377,158 | 40 | $7.6M | Research |
| Wha Three, LLC | Retail Trade | $374,830 | $379,082 | 32 | $8.1M | Research |
| Modern-Tec Manufacturing, Inc. | Wholesale Trade | $371,300 | $374,586 | 33 | $10.1M | Research |
| Comtec of W.n.y., Inc. | Construction | $367,876 | $369,902 | 48 | $15.8M | Research |
| R.a. Anzalone Electrical Contracting, Inc. | Construction | $336,535 | $339,975 | 54 | $17.8M | Research |
| Contracts Unlimited INC | Manufacturing | $332,470 | $334,237 | 38 | $9.3M | Research |
| Christopher E Beney Md, P.c. | Health Care and Social Assistance | $330,300 | $313,118 | 36 | $11.3M | Research |
| Eastern Niagara Medical Group P.c. | Health Care and Social Assistance | $325,000 | $134,271 | 8 | <$5M | Research |
| Great Lakes Asset Solutions LLC | Administrative and Support and Waste Management | $325,000 | $326,799 | 36 | $6.8M | Research |
| Desales Catholic School | Educational Services | $319,197 | $320,902 | 40 | N/A | Research |
| Christopher E Beney MD P.c. | Health Care and Social Assistance | $311,200 | $313,033 | 39 | $12.2M | Research |
| Custom Crews Inc. | Construction | $306,900 | $309,170 | 30 | $10.2M | Research |
| Mike Basil Automotive Group, LLC | Retail Trade | $304,627 | $307,373 | 27 | $6.8M | Research |
| E. A. Granchelli, Developer | Real Estate and Rental and Leasing | $301,304 | $68,539 | 7 | <$5M | Research |
| Cobbs Hill Manor INC | Health Care and Social Assistance | $300,900 | $304,272 | 60 | $10.2M | Research |
| De Sales Catholic School | Other Services (except Public Administration) | $300,697 | $302,551 | 41 | N/A | Research |
| Spire Recovery Solutions LLC | Administrative and Support and Waste Management | $297,672 | $0 | 88 | $16.6M | Research |
| E&r Machine Inc. | Manufacturing | $293,942 | $295,690 | 23 | $5.6M | Research |