| The Glockner Chevrolet Company | Retail Trade | $3,739,400 | $3,806,299 | 389 | $98.0M |  Research |
| Osco Industries INC | Manufacturing | $3,668,700 | $3,721,794 | 356 | $93.2M | Research |
| Portsmouth Emergency Ambulance Service INC | Health Care and Social Assistance | $3,629,900 | $3,672,148 | 389 | $90.1M | Research |
| Scioto County Counseling Center, Inc. | Health Care and Social Assistance | $3,372,730 | $3,410,767 | 322 | N/A | Research |
| Wendschmidt Wv Inc. | Accommodation and Food Services | $2,059,352 | $1,843,523 | 500 | $88.8M | Research |
| Community Action Organization of Scioto County INC | Other Services (except Public Administration) | $1,844,543 | $86,092 | 317 | N/A | Research |
| Shawnee Mental Health Center, Inc. | Health Care and Social Assistance | $1,498,100 | $1,512,915 | 158 | N/A | Research |
| Hill View Retirement Center | Health Care and Social Assistance | $1,376,700 | $1,393,220 | 199 | N/A | Research |
| Appalachian Wood Floors Inc. | Manufacturing | $1,298,309 | $1,315,525 | 190 | $188.5M | Research |
| Appalachian Wood Floors, Inc. | Manufacturing | $1,121,900 | $1,129,707 | 137 | $135.9M | Research |
| Mechanical Construction Company | Construction | $1,114,738 | $1,119,692 | 50 | $15.8M | Research |
| Jags, Inc. | Accommodation and Food Services | $1,017,800 | $893,688 | 363 | $64.5M | Research |
| Genesis Respiratory Services, INC | Retail Trade | $1,010,818 | $1,024,829 | 123 | $21.5M | Research |
| Lute Supply, Inc. | Wholesale Trade | $898,170 | $904,407 | 99 | $30.3M | Research |
| Mechanical Construction Company | Construction | $802,185 | $808,001 | 41 | $46.7M | Research |
| Sciotoville Community College | Educational Services | $716,407 | $721,820 | 82 | N/A | Research |
| Scioto Residential Services Inc. | Health Care and Social Assistance | $702,627 | $707,343 | 85 | $14.5M | Research |
| Jars I INC | Management of Companies and Enterprises | $622,642 | $631,615 | 216 | $35.5M | Research |
| Portsmouth Healthcare LLC | Health Care and Social Assistance | $601,219 | $607,841 | 88 | $15.0M | Research |
| West End Electric Co. Inc. | Construction | $587,423 | $590,572 | 60 | $19.7M | Research |
| Ct Ohio Portsmouth, LLC | Health Care and Social Assistance | $581,791 | $588,514 | 88 | $15.0M | Research |
| Jay Hash LLC | Health Care and Social Assistance | $541,100 | $543,772 | 62 | $10.5M | Research |
| Wendschmidt Ky, INC | Accommodation and Food Services | $511,965 | $453,980 | 238 | $42.3M | Research |
| Compass Community Health | Health Care and Social Assistance | $510,800 | $515,951 | 39 | N/A | Research |
| J & H Reinforcing & Structural Erectors | Construction | $472,515 | $475,232 | 40 | $45.6M | Research |