| Yankton Medical Clinic, P.c. | Health Care and Social Assistance | $4,096,900 | $4,144,042 | 264 | $52.4M |  Research |
| Shur-Co, LLC | Manufacturing | $3,016,000 | $3,050,374 | 315 | $82.5M | Research |
| Mount Marty College, INC | Educational Services | $1,700,000 | $1,712,156 | 249 | $29.9M | Research |
| Doug Marquardt Inc. | Transportation and Warehousing | $1,416,900 | $1,424,824 | 121 | $24.4M | Research |
| Lewis & Clark Behavioral Health, Inc. | Health Care and Social Assistance | $1,163,700 | $1,171,607 | 160 | $27.2M | Research |
| Ehresmann Engineering, Inc. | Professional, Scientific, and Technical Services | $585,700 | $588,990 | 44 | $5.4M | Research |
| Northern Extrusion Tooling INC | Manufacturing | $524,792 | $526,445 | 42 | $11.0M | Research |
| Northern Extrusion Tooling, INC | Manufacturing | $524,700 | $529,789 | 37 | $9.7M | Research |
| Ehresmann Engineering INC | Construction | $517,500 | $519,839 | 43 | $9.9M | Research |
| Mark's Machinery INC | Other Services (except Public Administration) | $502,500 | $506,878 | 38 | $8.2M | Research |
| Northtown, Inc. | Retail Trade | $467,800 | $472,158 | 49 | $12.3M | Research |
| Riverfront Broadcasting LLC | Information | $454,750 | $457,067 | 55 | $9.2M | Research |
| Solid Metals Manufacturing LLC | Manufacturing | $447,600 | $451,426 | 29 | $7.1M | Research |
| Solid Metals Manufacturing LLC | Manufacturing | $413,150 | $415,131 | 28 | $6.8M | Research |
| Riverfront Broadcasting, LLC | Information | $389,200 | $392,857 | 37 | $6.2M | Research |
| Electair, Inc. | Manufacturing | $363,800 | $366,661 | 32 | $8.4M | Research |
| Ability Building Services, Inc. | Health Care and Social Assistance | $360,500 | $363,186 | 120 | N/A | Research |
| Meridian Liquids Partners, LLC | Wholesale Trade | $348,800 | $352,670 | 23 | $5.9M | Research |
| Slowey Management LLC | Administrative and Support and Waste Management | $333,300 | $335,902 | 20 | <$5M | Research |
| Larry's Heating and Cooling, Inc. | Construction | $323,397 | $325,589 | 22 | $7.0M | Research |
| Fryn' Pan of Yankton | Accommodation and Food Services | $304,800 | $306,358 | 30 | $5.3M | Research |
| Slowey Management LLC | Administrative and Support and Waste Management | $296,522 | $298,269 | 16 | <$5M | Research |
| Stockmen's Livestock Inc. | Agriculture, Forestry, Fishing and Hunting | $282,815 | $284,760 | 66 | <$5M | Research |
| Sacred Heart School | Educational Services | $276,200 | $278,289 | 45 | N/A | Research |
| Benedictine Convent of the Sacred Heart | Other Services (except Public Administration) | $273,900 | $275,949 | 69 | N/A | Research |