| The Road Guy Construction Company, Inc. | Construction | $269,100 | $270,924 | 13 | <$5M | Research |
| Fair Manufacturing INC | Manufacturing | $264,362 | $265,485 | 21 | $5.5M | Research |
| First Chiropractic | Health Care and Social Assistance | $259,600 | $260,561 | 21 | <$5M | Research |
| Slowey Construction, INC | Construction | $258,100 | $259,605 | 16 | <$5M | Research |
| Pro Windmill, Inc. | Administrative and Support and Waste Management | $257,500 | $259,080 | 22 | <$5M | Research |
| Lewis & Clark Ford, Lincoln, Mercury, Inc. | Retail Trade | $256,900 | $259,195 | 18 | <$5M | Research |
| Chris Dickes Family Dentistry Prof LLC | Health Care and Social Assistance | $256,000 | $257,964 | 18 | <$5M | Research |
| Stockmen's Livestock INC | Agriculture, Forestry, Fishing and Hunting | $247,200 | $249,090 | 87 | <$5M | Research |
| Broadway Chrysler Dodge Jeep INC | Retail Trade | $240,600 | $242,933 | 19 | <$5M | Research |
| The Road Guy Construction Company Inc. | Construction | $240,534 | $241,563 | 24 | $7.4M | Research |
| Fryn' Pan of Yankton, INC | Accommodation and Food Services | $240,200 | $241,760 | 50 | $8.9M | Research |
| Willcockson Eye Associates PC | Health Care and Social Assistance | $231,765 | $232,921 | 21 | <$5M | Research |
| Fair Manufacturing Inc. | Manufacturing | $230,000 | $231,439 | 21 | $5.5M | Research |
| Wohlenberg Ritzman & Co., LLC | Professional, Scientific, and Technical Services | $228,000 | $229,524 | 14 | <$5M | Research |
| Ymker Insulation INC | Construction | $226,208 | $227,819 | 16 | $5.5M | Research |
| Yaggie's INC | Agriculture, Forestry, Fishing and Hunting | $225,800 | $227,062 | 16 | <$5M | Research |
| Willcockson Eye Associates, P.c. | Health Care and Social Assistance | $218,000 | $219,421 | 21 | <$5M | Research |
| Wohlenberg Ritzman & Co LLC | Professional, Scientific, and Technical Services | $212,384 | $213,484 | 14 | <$5M | Research |
| Yankton Media INC | Wholesale Trade | $205,955 | $207,357 | 23 | $5.9M | Research |
| Topkote, Inc. | Construction | $196,065 | $197,596 | 17 | $5.3M | Research |
| Elliot Construction INC | Construction | $188,300 | $189,698 | 15 | $5.6M | Research |
| Yankton Anesthesiology, PC | Health Care and Social Assistance | $173,700 | $154,547 | 5 | <$5M | Research |
| Dayhuff Enterprises, Inc. | Accommodation and Food Services | $166,500 | $167,827 | 47 | $5.8M | Research |
| John A. Conkling Distributing Company, Inc. | Transportation and Warehousing | $155,500 | $198,639 | 16 | <$5M | Research |
| Kopetsky Enterprises INC | Retail Trade | $152,800 | $153,868 | 19 | <$5M | Research |