| Shelor Motor Mile INC | Retail Trade | $5,457,500 | $5,473,228 | 444 | $111.9M |  Research |
| Gillmann Services INC | Administrative and Support and Waste Management | $5,175,300 | $5,243,480 | 408 | $64.2M | Research |
| Community Housing Partners Corp | Health Care and Social Assistance | $4,278,800 | $4,006,074 | 340 | N/A | Research |
| Twenty 20 Management, Inc. | Health Care and Social Assistance | $2,343,500 | $2,371,882 | 398 | $67.7M | Research |
| Gillmann Services Inc. | Administrative and Support and Waste Management | $2,000,000 | $0 | 295 | $46.4M | Research |
| Twenty 20 Management Inc. | Health Care and Social Assistance | $1,684,080 | $1,692,734 | 215 | $36.6M | Research |
| Virginia Community Capital, Inc. | Finance and Insurance | $1,005,933 | $1,016,571 | 65 | N/A | Research |
| Turman Lumber Co., Inc. | Manufacturing | $937,000 | $943,273 | 100 | $99.2M | Research |
| Valley Landscaping, Inc. | Administrative and Support and Waste Management | $789,900 | $794,618 | 175 | $37.9M | Research |
| Free Clinic of the New River Valley, Inc. | Health Care and Social Assistance | $745,500 | $750,159 | 71 | N/A | Research |
| Justin Yalung | Manufacturing | $698,600 | $688,801 | 71 | $70.4M | Research |
| Blue Ridge Heating & Air, Inc. | Construction | $619,950 | $626,718 | 40 | $12.7M | Research |
| Duncan Enterprises Company Va | Retail Trade | $561,105 | $568,653 | 47 | $11.8M | Research |
| Shah Development, LLC | Real Estate and Rental and Leasing | $512,000 | $0 | 50 | $7.5M | Research |
| Rogue Staffing INC | Administrative and Support and Waste Management | $492,900 | $497,370 | 50 | $7.9M | Research |
| Town & Country Veterinary Clinic, P.c. | Professional, Scientific, and Technical Services | $484,000 | $487,334 | 50 | $7.0M | Research |
| Bill's Used Auto Parts | Wholesale Trade | $440,300 | $443,726 | 48 | $14.7M | Research |
| Duncan Imports INC | Retail Trade | $396,225 | $401,425 | 34 | $8.6M | Research |
| Mcgrady-Perdue Heating and Cooling INC | Construction | $371,757 | $373,682 | 33 | $10.4M | Research |
| First Team Nissan Christainsburg, LLC | Retail Trade | $362,700 | $366,962 | 35 | $8.8M | Research |
| Mcgrady-Perdue Heating and Cooling Inc. | Construction | $346,100 | $348,357 | 38 | $12.0M | Research |
| Milestone Development, LLC | Accommodation and Food Services | $345,500 | $347,809 | 88 | $27.5M | Research |
| Macon Subway Dough Inc. | Accommodation and Food Services | $343,910 | $346,869 | 42 | $7.5M | Research |
| Montgomery Restaurant Corporation | Accommodation and Food Services | $327,487 | $331,935 | 42 | $7.5M | Research |
| Anesthesiology Assoc. of Radford | Health Care and Social Assistance | $308,333 | $312,256 | 10 | <$5M | Research |