| Virginia Techniques Gymnastics, Inc. | Arts, Entertainment, and Recreation | $151,000 | $152,009 | 22 | <$5M |  Research |
| Graceway Ministries Graceachildusa, Inc. | Health Care and Social Assistance | $150,400 | $151,850 | 32 | $5.2M | Research |
| J.r.a.f., Inc. | Transportation and Warehousing | $150,400 | $151,628 | 22 | $8.5M | Research |
| Virginia Techniques Gymnastics Inc. | Educational Services | $149,465 | $150,452 | 45 | $5.8M | Research |
| Hall's Garage Doors | Retail Trade | $145,000 | $146,631 | 15 | <$5M | Research |
| Alvis Laing Electric Co | Construction | $142,890 | $146,502 | 12 | <$5M | Research |
| Koeda Corp | Accommodation and Food Services | $142,800 | $144,403 | 28 | <$5M | Research |
| Meld Manufacturing Corporation | Manufacturing | $142,655 | $144,058 | 10 | <$5M | Research |
| Altis Enterprises LLC | Real Estate and Rental and Leasing | $142,400 | $144,018 | 30 | <$5M | Research |
| Dvd Masonry LLC | Construction | $142,000 | $143,047 | 20 | <$5M | Research |
| Douloi Inc. DBA Home Instead Senoir Care | Health Care and Social Assistance | $140,928 | $142,427 | 25 | <$5M | Research |
| Alvis-Laing Electric Co. | Construction | $140,340 | $141,022 | 16 | $5.3M | Research |
| Country Kitchen of Christiansburg INC | Accommodation and Food Services | $132,853 | $133,532 | 23 | <$5M | Research |
| Prestar Packaging, Inc. | Administrative and Support and Waste Management | $132,000 | $133,291 | 19 | <$5M | Research |
| Habitat for Humanity Inc, of the New River Valley | Other Services (except Public Administration) | $130,400 | $131,658 | 18 | <$5M | Research |
| Habitat for Humanity INC of the New River Valley | Health Care and Social Assistance | $130,400 | $131,325 | 19 | <$5M | Research |
| Christiansburg Pharmacy Inc. | Retail Trade | $130,100 | $130,934 | 15 | <$5M | Research |
| Alston Enterprises Inc. | Accommodation and Food Services | $127,932 | $128,640 | 30 | $5.3M | Research |
| Show-Case Shutters Inc. | Retail Trade | $126,100 | $127,320 | 20 | <$5M | Research |
| Virginia Children's Dentistry, Pllc | Health Care and Social Assistance | $125,500 | $67,936 | 13 | <$5M | Research |
| Customized Lawn Care INC | Administrative and Support and Waste Management | $122,312 | $122,975 | 16 | <$5M | Research |
| Lancaster Investments Inc. | Other Services (except Public Administration) | $120,400 | $120,769 | 11 | <$5M | Research |
| Customized Lawn Care, INC | Administrative and Support and Waste Management | $120,100 | $121,314 | 16 | <$5M | Research |
| Prestar Packaging | Manufacturing | $120,000 | $120,667 | 19 | $18.8M | Research |
| Lancaster Investments INC | Other Services (except Public Administration) | $118,200 | $119,382 | 14 | <$5M | Research |