| Dunlop House, LLC | Health Care and Social Assistance | $662,400 | $668,049 | 88 | $15.0M |  AI Deep Dive |
| Roanoke Catholic School | Educational Services | $660,073 | $666,362 | 80 | N/A | AI Deep Dive |
| Stafford Operations, LLC | Health Care and Social Assistance | $657,790 | $669,522 | 151 | $25.7M | AI Deep Dive |
| Harbour View Harmony Management, LLC | Health Care and Social Assistance | $650,268 | $657,643 | 98 | $16.7M | AI Deep Dive |
| Excel Prosthetics and Orthotics | Manufacturing | $638,800 | $642,349 | 49 | $13.7M | AI Deep Dive |
| Dominion Air & Machinery Co | Wholesale Trade | $635,200 | $639,346 | 52 | $15.9M | AI Deep Dive |
| New Hope Support Services, LLC | Health Care and Social Assistance | $632,000 | $640,497 | 87 | $20.4M | AI Deep Dive |
| Physicians to Women, Inc. | Health Care and Social Assistance | $631,500 | $639,095 | 61 | $19.1M | AI Deep Dive |
| F&w Management, Lc | Real Estate and Rental and Leasing | $624,000 | $277,585 | 35 | $5.3M | AI Deep Dive |
| S Lewis Lionberger Construction Company | Construction | $620,860 | $625,879 | 49 | $55.8M | AI Deep Dive |
| Endocrinology Associates, Inc. | Health Care and Social Assistance | $608,600 | $616,253 | 54 | $16.9M | AI Deep Dive |
| Shively Electrical Co INC | Construction | $607,100 | $612,631 | 64 | $21.1M | AI Deep Dive |
| Unified Human Services Transportation Systems, Inc. | Transportation and Warehousing | $601,023 | $607,955 | 98 | $30.1M | AI Deep Dive |
| Foti, Flynn, Lowen & Company, a Professional Corporation | Professional, Scientific, and Technical Services | $599,910 | $607,359 | 42 | $13.4M | AI Deep Dive |
| Potomac Place Associates | Health Care and Social Assistance | $587,293 | $592,635 | 77 | $13.1M | AI Deep Dive |
| Frith Anderson & Peake PC | Professional, Scientific, and Technical Services | $580,500 | $584,031 | 31 | $7.5M | AI Deep Dive |
| National Pools of Roanoke INC | Wholesale Trade | $576,100 | $579,893 | 49 | $15.0M | AI Deep Dive |
| Roanoke Valley Pizza, Inc. | Accommodation and Food Services | $575,000 | $232,388 | 61 | $10.8M | AI Deep Dive |
| Rescue Mission of Roaoke, Inc. | Health Care and Social Assistance | $571,797 | $574,862 | 93 | N/A | AI Deep Dive |
| Lee Hartman and Sons INC | Retail Trade | $570,700 | $577,580 | 69 | $21.1M | AI Deep Dive |
| First Four Petroleum Group LLC | Retail Trade | $569,800 | $575,118 | 37 | $8.8M | AI Deep Dive |
| Blue Ridge Color Company, Inc. | Wholesale Trade | $565,100 | $568,396 | 47 | $14.4M | AI Deep Dive |
| Hanover Operations, LLC | Health Care and Social Assistance | $563,780 | $570,206 | 113 | $19.2M | AI Deep Dive |
| Unified Human Services Transportation Systems Inc. | Transportation and Warehousing | $558,935 | $564,019 | 92 | $28.3M | AI Deep Dive |
| West Ashley Operations, LLC | Health Care and Social Assistance | $557,418 | $563,755 | 112 | $19.1M | AI Deep Dive |