| Allegheny Construction Company Inc. | Construction | $484,600 | $487,481 | 47 | $14.6M | AI Deep Dive |
| Milestone Development LLC | Accommodation and Food Services | $483,700 | $488,683 | 88 | $27.5M | AI Deep Dive |
| Kennedy Business Services Ltd | Professional, Scientific, and Technical Services | $477,400 | $480,052 | 30 | <$5M | AI Deep Dive |
| Precision Steel Manfacturing Corporation | Manufacturing | $476,000 | $479,456 | 38 | $9.3M | AI Deep Dive |
| Avis Construction Company, Inc. | Construction | $475,275 | $481,056 | 47 | $53.5M | AI Deep Dive |
| Murfreesboro Operations, LLC | Health Care and Social Assistance | $474,473 | $479,867 | 113 | $19.2M | AI Deep Dive |
| Greenville Operations, LLC | Health Care and Social Assistance | $468,993 | $474,312 | 80 | $13.6M | AI Deep Dive |
| Precision Steel Manufacturing Corporation | Manufacturing | $464,500 | $467,093 | 32 | $7.8M | AI Deep Dive |
| Physician Associates of Virginia PC | Health Care and Social Assistance | $460,700 | $463,780 | 49 | $15.4M | AI Deep Dive |
| Chesapeake Operations, LLC | Health Care and Social Assistance | $458,703 | $463,905 | 120 | $20.4M | AI Deep Dive |
| Wagner Food Equipment INC | Retail Trade | $455,382 | $458,304 | 28 | $6.7M | AI Deep Dive |
| Gastroenterology Consultants of Southwest Virginia, Inc. | Health Care and Social Assistance | $454,642 | $457,166 | 35 | $6.2M | AI Deep Dive |
| Mountain Roofing Inc. | Construction | $444,636 | $447,662 | 40 | $13.2M | AI Deep Dive |
| Butler Parachute Systems, Inc. | Manufacturing | $443,948 | $447,098 | 44 | $15.5M | AI Deep Dive |
| Bb&c Enterprises, LLC | Accommodation and Food Services | $443,100 | $446,091 | 152 | $27.0M | AI Deep Dive |
| Ib Operations, LLC | Health Care and Social Assistance | $440,410 | $445,417 | 84 | $14.3M | AI Deep Dive |
| National University Services INC | Educational Services | $434,600 | $437,743 | 45 | $5.4M | AI Deep Dive |
| Roanoke Ambulatory Surgery Center L.l.c. | Health Care and Social Assistance | $426,500 | $430,099 | 34 | $6.1M | AI Deep Dive |
| Roanoke Ambulatory Surgery Center L.l.c. | Health Care and Social Assistance | $426,500 | $429,491 | 38 | $6.8M | AI Deep Dive |
| Accordius Health at Roanoke LLC | Health Care and Social Assistance | $425,563 | $430,777 | 62 | $10.5M | AI Deep Dive |
| Morgantown Operations, LLC | Health Care and Social Assistance | $424,338 | $429,151 | 73 | $12.4M | AI Deep Dive |
| Harkness Screens (usa) Ltd | Manufacturing | $424,202 | $431,849 | 19 | <$5M | AI Deep Dive |
| Prmc Operations, LLC | Unknown | $423,970 | $428,698 | 86 | N/A | AI Deep Dive |
| Clarke Investments LLC | Management of Companies and Enterprises | $422,363 | $0 | 38 | $6.2M | AI Deep Dive |
| Automotive Ventures INC | Retail Trade | $421,003 | $426,678 | 34 | $8.6M | AI Deep Dive |