| Bridgeport Operations, LLC | Health Care and Social Assistance | $369,380 | $373,499 | 48 | $8.2M | AI Deep Dive |
| Security Scale Service INC | Wholesale Trade | $367,855 | $369,730 | 30 | $9.2M | AI Deep Dive |
| Network Computing Group Inc. | Professional, Scientific, and Technical Services | $365,585 | $367,809 | 21 | <$5M | AI Deep Dive |
| Network Computing Group, Inc. | Professional, Scientific, and Technical Services | $365,585 | $368,770 | 21 | <$5M | AI Deep Dive |
| H.l. Lawson & Sons, Inc. | Transportation and Warehousing | $364,000 | $366,558 | 30 | $7.6M | AI Deep Dive |
| Kbs Analytics LLC | Professional, Scientific, and Technical Services | $360,977 | $363,321 | 23 | <$5M | AI Deep Dive |
| Dominion Pathology Associates PC | Health Care and Social Assistance | $360,520 | $363,544 | 14 | <$5M | AI Deep Dive |
| S. C. Rossi & Company Inc. | Construction | $359,598 | $361,499 | 22 | $7.5M | AI Deep Dive |
| S. C. Rossi & Company, Inc. | Construction | $359,598 | $361,765 | 31 | $10.6M | AI Deep Dive |
| Spectrum Design PC | Professional, Scientific, and Technical Services | $358,100 | $359,950 | 24 | <$5M | AI Deep Dive |
| 1912 Grill INC | Accommodation and Food Services | $355,186 | $358,244 | 54 | $9.6M | AI Deep Dive |
| Hampden Operations, LLC | Health Care and Social Assistance | $352,698 | $356,698 | 75 | $12.8M | AI Deep Dive |
| Webb's Oil Company Incorporated | Wholesale Trade | $352,000 | $353,969 | 27 | $6.9M | AI Deep Dive |
| Harris Office Furniture Co. Inc. | Wholesale Trade | $351,800 | $353,875 | 24 | $7.4M | AI Deep Dive |
| Justice Energy Company INC | Mining, Quarrying, and Oil and Gas Extraction | $349,723 | $0 | 29 | <$5M | AI Deep Dive |
| Emergency Veterinary Service of Roanoke Inc. | Professional, Scientific, and Technical Services | $348,675 | $352,018 | 44 | $6.2M | AI Deep Dive |
| Leisure Media360 INC | Information | $348,300 | $352,547 | 27 | <$5M | AI Deep Dive |
| Webb's Oil Company Incorporated | Wholesale Trade | $347,600 | $350,190 | 27 | $6.9M | AI Deep Dive |
| Girl Scouts of Virginia Skyline Council, Inc. | Other Services (except Public Administration) | $344,840 | $349,246 | 29 | N/A | AI Deep Dive |
| Allergy & Immunology Plc | Health Care and Social Assistance | $343,800 | $345,806 | 26 | $8.2M | AI Deep Dive |
| Georg North America INC | Accommodation and Food Services | $343,160 | $346,639 | 20 | $6.3M | AI Deep Dive |
| Pitman Construction Company INC | Construction | $339,700 | $342,720 | 33 | $10.0M | AI Deep Dive |
| First Baptist Church of Roanoke INC | Other Services (except Public Administration) | $336,480 | $338,610 | 61 | N/A | AI Deep Dive |
| Joe Bandy and Son Inc. | Construction | $335,700 | $375,776 | 53 | $19.9M | AI Deep Dive |
| Acceleration Point, LLC | Professional, Scientific, and Technical Services | $335,140 | $337,011 | 20 | <$5M | AI Deep Dive |