| Roanoke Airport Hotel Partners LLC | Accommodation and Food Services | $334,032 | $335,962 | 29 | $9.1M | AI Deep Dive |
| Dominion Metallurgical Inc. | Professional, Scientific, and Technical Services | $333,715 | $336,595 | 20 | <$5M | AI Deep Dive |
| Harris Office Furniture Co INC | Retail Trade | $332,227 | $334,377 | 24 | <$5M | AI Deep Dive |
| Hall Community Services, Inc. | Health Care and Social Assistance | $330,708 | $333,317 | 50 | $8.1M | AI Deep Dive |
| Lucas & Kite, Plc | Professional, Scientific, and Technical Services | $330,600 | $332,427 | 21 | $5.1M | AI Deep Dive |
| Justin Roanoke Hotel LLC | Accommodation and Food Services | $329,563 | $332,164 | 47 | $14.7M | AI Deep Dive |
| Custom Tool and Machine, Inc. | Manufacturing | $329,000 | $332,692 | 32 | $7.8M | AI Deep Dive |
| Blue Ridge Mountains Council INC | Other Services (except Public Administration) | $322,998 | $325,232 | 11 | <$5M | AI Deep Dive |
| Blue Ridge Mountains Council, Boy Scouts of America, Inc. | Other Services (except Public Administration) | $322,900 | $327,017 | 193 | $36.9M | AI Deep Dive |
| Bower Heating and Air Conditioning INC | Construction | $322,160 | $324,308 | 30 | $9.5M | AI Deep Dive |
| Richard D. Smith II DDS P.c. | Health Care and Social Assistance | $320,631 | $322,956 | 35 | $8.1M | AI Deep Dive |
| Stoner Quality Water, INC | Construction | $318,195 | $320,706 | 34 | $10.8M | AI Deep Dive |
| Valley Machine Co. Inc. | Manufacturing | $315,760 | $317,128 | 50 | $12.2M | AI Deep Dive |
| American National University of Kentucky Inc. | Educational Services | $315,500 | $319,597 | 28 | <$5M | AI Deep Dive |
| Cave Spring Auto Body, Inc. | Other Services (except Public Administration) | $313,738 | $315,568 | 22 | <$5M | AI Deep Dive |
| Cave Spring Auto Body Inc. | Other Services (except Public Administration) | $313,737 | $315,190 | 22 | <$5M | AI Deep Dive |
| Virginia Interventional Pain & Spine Center Inc. | Health Care and Social Assistance | $311,400 | $315,016 | 25 | $7.8M | AI Deep Dive |
| Virginia Interventional Pain | Health Care and Social Assistance | $308,910 | $311,544 | 25 | $7.8M | AI Deep Dive |
| Glenn, Feldmann, Darby & Goodlatte, Aprofessional Corporatio | Professional, Scientific, and Technical Services | $307,400 | $309,390 | 19 | <$5M | AI Deep Dive |
| Moss & Rocovich, Attorneys-At-Law, P.c. | Professional, Scientific, and Technical Services | $303,000 | $306,894 | 18 | <$5M | AI Deep Dive |
| Assisi Animal Clinics of Virginia | Other Services (except Public Administration) | $302,700 | $304,382 | 44 | N/A | AI Deep Dive |
| Berglund Management Group INC | Retail Trade | $301,600 | $305,412 | 17 | <$5M | AI Deep Dive |
| Peninsula Pathology Associates INC | Health Care and Social Assistance | $301,200 | $304,421 | 8 | <$5M | AI Deep Dive |
| Dominion Metallurgical, Inc. | Manufacturing | $300,035 | $302,352 | 20 | <$5M | AI Deep Dive |
| Crawford Environmental Services, LLC | Construction | $295,200 | $296,793 | 40 | $15.0M | AI Deep Dive |