| United Support Services, Llc. | Health Care and Social Assistance | $294,100 | $278,611 | 31 | $7.3M |  AI Deep Dive |
| Taubman Musuem of Art | Arts, Entertainment, and Recreation | $293,805 | $295,070 | 31 | N/A | AI Deep Dive |
| B&b Machine & Tool Inc. | Construction | $291,100 | $294,116 | 38 | $12.1M | AI Deep Dive |
| Montano's International Gourmet Shop's Inc. | Accommodation and Food Services | $288,800 | $290,685 | 62 | $11.0M | AI Deep Dive |
| Kbs Analytics, LLC | Professional, Scientific, and Technical Services | $288,258 | $290,090 | 15 | <$5M | AI Deep Dive |
| Rubicon Planning, LLC | Professional, Scientific, and Technical Services | $287,300 | $289,694 | 14 | <$5M | AI Deep Dive |
| General Sales of Virginia, Inc. | Wholesale Trade | $286,607 | $288,303 | 28 | $8.6M | AI Deep Dive |
| Sj Conner & Sons INC | Construction | $285,986 | $288,493 | 30 | $9.5M | AI Deep Dive |
| Intoprint Technologies, Inc. | Wholesale Trade | $285,800 | $289,418 | 26 | $8.0M | AI Deep Dive |
| Stage Sound INC | Manufacturing | $285,771 | $288,668 | 29 | $7.6M | AI Deep Dive |
| Dehaven Transportation Company, INC | Transportation and Warehousing | $284,100 | $286,515 | 41 | $6.2M | AI Deep Dive |
| Lucas Physical Therapy INC | Health Care and Social Assistance | $281,200 | $283,012 | 30 | $8.1M | AI Deep Dive |
| Weed Control, Inc. | Agriculture, Forestry, Fishing and Hunting | $281,000 | $283,053 | 35 | <$5M | AI Deep Dive |
| Hampton Il Operations, LLC | Health Care and Social Assistance | $280,778 | $283,962 | 60 | $10.2M | AI Deep Dive |
| United Way of Roanoke Valley Inc. | Other Services (except Public Administration) | $279,800 | $281,541 | 31 | N/A | AI Deep Dive |
| Mid-Atlantic Heating & Cooling, Inc. | Construction | $278,638 | $281,393 | 27 | $8.5M | AI Deep Dive |
| Transformed Leadership LLC | Health Care and Social Assistance | $277,613 | $281,105 | 35 | $8.2M | AI Deep Dive |
| Mcleod Enterprises LP | Professional, Scientific, and Technical Services | $277,487 | $280,323 | 20 | $6.4M | AI Deep Dive |
| Bondough Inc. | Accommodation and Food Services | $276,892 | $280,838 | 35 | $6.2M | AI Deep Dive |
| Varsity Landscaping & Grounds LLC | Administrative and Support and Waste Management | $276,482 | $279,055 | 26 | $5.6M | AI Deep Dive |
| Benny Marzanos | Accommodation and Food Services | $275,800 | $277,302 | 35 | $6.2M | AI Deep Dive |
| Falletta Enterprises, Inc. | Finance and Insurance | $275,000 | $278,239 | 25 | $11.0M | AI Deep Dive |
| Family Service of Roanoke Valley | Health Care and Social Assistance | $274,100 | $275,889 | 43 | N/A | AI Deep Dive |
| The Roanoke Country Club Incorporated | Arts, Entertainment, and Recreation | $273,404 | $275,149 | 99 | $13.9M | AI Deep Dive |
| Basham Oil Inc. | Wholesale Trade | $272,838 | $276,695 | 62 | $19.0M | AI Deep Dive |