| Mario Industries of Virginia, Inc. | Manufacturing | $251,265 | $252,614 | 26 | $6.8M |  AI Deep Dive |
| Bonsack Baptist Church | Other Services (except Public Administration) | $251,028 | $252,712 | 55 | N/A | AI Deep Dive |
| Certified Medical Representatives Institute INC | Educational Services | $250,878 | $252,929 | 15 | <$5M | AI Deep Dive |
| Gbsr Attorneys, LLP | Professional, Scientific, and Technical Services | $250,295 | $251,730 | 20 | <$5M | AI Deep Dive |
| Gilbert Bird Sharpes and Robinson | Professional, Scientific, and Technical Services | $250,200 | $252,202 | 19 | <$5M | AI Deep Dive |
| Roanoke Glass Incorporated | Construction | $249,200 | $250,723 | 24 | $5.6M | AI Deep Dive |
| Blue Ridge Public Television, Inc. | Information | $248,700 | $183,277 | 18 | <$5M | AI Deep Dive |
| Bug Man Exterminating, Inc. | Administrative and Support and Waste Management | $248,300 | $250,142 | 21 | <$5M | AI Deep Dive |
| Lumsden Associates, P.c. | Professional, Scientific, and Technical Services | $247,625 | $249,035 | 20 | <$5M | AI Deep Dive |
| Varsity Landscaping & Grounds LLC | Administrative and Support and Waste Management | $247,417 | $248,908 | 27 | $5.8M | AI Deep Dive |
| Black Dog Salvage LLC | Retail Trade | $247,200 | $249,239 | 25 | <$5M | AI Deep Dive |
| Black Dog Salvage, LLC | Wholesale Trade | $247,200 | $249,970 | 25 | $7.7M | AI Deep Dive |
| Glenn Robinson Cathey Memmer & Skaff Plc | Professional, Scientific, and Technical Services | $246,900 | $248,306 | 19 | <$5M | AI Deep Dive |
| 202 Social House INC | Accommodation and Food Services | $246,832 | $249,040 | 49 | $8.7M | AI Deep Dive |
| Habitat for Humanity in the Roanoke Valley, Inc. | Health Care and Social Assistance | $243,040 | $244,647 | 24 | N/A | AI Deep Dive |
| American National University of Kentucky Inc. | Educational Services | $242,900 | $244,664 | 37 | <$5M | AI Deep Dive |
| Walker Machine and Foundry Corp | Manufacturing | $240,914 | $244,016 | 8 | <$5M | AI Deep Dive |
| Keltron of Virginia, Inc. | Administrative and Support and Waste Management | $240,900 | $242,499 | 13 | <$5M | AI Deep Dive |
| National O&m INC | Utilities | $240,160 | $0 | 34 | N/A | AI Deep Dive |
| National O&m INC | Utilities | $240,100 | $289,442 | 34 | N/A | AI Deep Dive |
| Western Sizzlin Franchise Corp | Unknown | $239,135 | $0 | 21 | N/A | AI Deep Dive |
| Valley Boiler & Mechanical Inc. | Manufacturing | $238,500 | $240,077 | 26 | $6.8M | AI Deep Dive |
| Dawson Truck Lines | Transportation and Warehousing | $237,848 | $240,491 | 9 | <$5M | AI Deep Dive |
| Dawson Truck Lines | Transportation and Warehousing | $237,798 | $240,671 | 11 | <$5M | AI Deep Dive |
| Brambleton Imports Inc. | Retail Trade | $237,700 | $239,047 | 21 | $5.3M | AI Deep Dive |