| Thompson & Litton Inc. | Professional, Scientific, and Technical Services | $1,530,700 | $1,539,129 | 131 | $16.2M |  Research |
| In Home Care Inc. | Health Care and Social Assistance | $458,400 | $461,427 | 141 | $24.2M | Research |
| Lifecare Ambulance Service INC | Health Care and Social Assistance | $452,900 | $456,734 | 50 | $11.6M | Research |
| In Home Care, INC | Health Care and Social Assistance | $435,300 | $438,651 | 105 | $18.0M | Research |
| Appalachian Technical Services Iinc | Professional, Scientific, and Technical Services | $426,976 | $430,748 | 29 | $9.0M | Research |
| Appalachian Technical Services Inc. | Professional, Scientific, and Technical Services | $426,976 | $429,170 | 25 | <$5M | Research |
| C S & S Coal Corporation | Mining, Quarrying, and Oil and Gas Extraction | $352,617 | $355,042 | 24 | <$5M | Research |
| J.w. Construction Co, Inc. | Mining, Quarrying, and Oil and Gas Extraction | $338,116 | $342,070 | 28 | <$5M | Research |
| Falcon Coal Corporation | Mining, Quarrying, and Oil and Gas Extraction | $332,355 | $334,331 | 23 | <$5M | Research |
| Falcon Coal Corporation | Mining, Quarrying, and Oil and Gas Extraction | $332,300 | $334,986 | 23 | <$5M | Research |
| Freedom Ford of Wise, Inc. | Retail Trade | $326,200 | $328,058 | 38 | $9.6M | Research |
| C. S. & S. Coal Corporation | Mining, Quarrying, and Oil and Gas Extraction | $315,500 | $318,223 | 23 | <$5M | Research |
| St Marys Health Wagon INC | Health Care and Social Assistance | $313,200 | $317,219 | 30 | N/A | Research |
| Reno's of Wise LLC | Accommodation and Food Services | $197,300 | $199,262 | 60 | $10.7M | Research |
| The Lee Law Firm of Wise, PC | Professional, Scientific, and Technical Services | $180,300 | $120,150 | 11 | <$5M | Research |
| Boggs Municipal Services, Inc. | Construction | $179,100 | $180,662 | 20 | $22.8M | Research |
| Newport Wise Management, LLC | Accommodation and Food Services | $166,800 | $168,588 | 6 | <$5M | Research |
| Renos of Wise LLC | Accommodation and Food Services | $140,800 | $141,953 | 55 | $9.8M | Research |
| Barnette Contractors, L.l.c. | Construction | $134,900 | $135,968 | 11 | $12.5M | Research |
| Barnette Contractors L.l.c. | Construction | $134,000 | $134,760 | 10 | $11.4M | Research |
| The Lee Law Firm of Wise, PC | Professional, Scientific, and Technical Services | $126,122 | $127,208 | 11 | <$5M | Research |
| Cavalier Pharmacy, Inc. | Retail Trade | $121,600 | $122,819 | 9 | <$5M | Research |
| Cavalier Pharmacy Inc. | Retail Trade | $121,043 | $123,378 | 9 | <$5M | Research |
| Friendship Home Health, Inc. | Retail Trade | $108,500 | $109,492 | 12 | <$5M | Research |
| Rigg Properties, Inc. | Real Estate and Rental and Leasing | $107,700 | $108,541 | 6 | <$5M | Research |