| Opequon Motors | Retail Trade | $219,337 | $222,707 | 21 | $5.3M |  Research |
| Martinsburg Eye Associates, P.l.l.c. | Health Care and Social Assistance | $217,274 | $147,622 | 12 | <$5M | Research |
| Abbster Enterprises, LLC | Accommodation and Food Services | $217,061 | $218,412 | 58 | $10.3M | Research |
| W Harley Miller Contractors Inc. | Construction | $213,119 | $214,877 | 19 | $21.6M | Research |
| W Harley Miller Contractors, Inc. | Construction | $213,100 | $214,698 | 19 | $21.6M | Research |
| American Family Matters, LLC | Finance and Insurance | $211,827 | $212,953 | 30 | $15.4M | Research |
| Martinsburg's Smallwood & Small Insurance, Inc. | Finance and Insurance | $207,400 | $153,624 | 13 | $6.7M | Research |
| Quinn Transportation, LLC | Transportation and Warehousing | $203,100 | $163,071 | 13 | <$5M | Research |
| Country Roads Tire & Auto | Other Services (except Public Administration) | $197,900 | $199,914 | 17 | <$5M | Research |
| Boyd & Stephen-Hassard DDS Pllc | Health Care and Social Assistance | $197,564 | $199,353 | 14 | <$5M | Research |
| Center for Dermatology-Erik B Hurst, Pllc | Health Care and Social Assistance | $195,200 | $196,534 | 18 | $5.6M | Research |
| Boyd & Stephen-Hassard DDS Pllc | Health Care and Social Assistance | $192,200 | $194,613 | 14 | <$5M | Research |
| Burke, Schultz, Harmon, Jenkinson | Professional, Scientific, and Technical Services | $191,200 | $193,909 | 11 | <$5M | Research |
| Martinsburg Radiology | Health Care and Social Assistance | $190,000 | $192,349 | 11 | <$5M | Research |
| Heritage Roofing INC | Construction | $185,187 | $187,810 | 14 | <$5M | Research |
| Fargo Insurance & Financial Services Inc. | Finance and Insurance | $184,000 | $186,263 | 18 | $9.2M | Research |
| Kidney Specialists Pllc | Health Care and Social Assistance | $183,500 | $185,722 | 9 | <$5M | Research |
| Terrasimco, Inc. | Manufacturing | $181,200 | $182,530 | 13 | <$5M | Research |
| Thomas J Meals Dmd Pllc | Health Care and Social Assistance | $179,500 | $181,807 | 15 | <$5M | Research |
| Patient Transportation Inc. | Transportation and Warehousing | $175,200 | $177,760 | 30 | $9.2M | Research |
| Johnson & Johnson Htg & A/c INC | Construction | $173,900 | $175,629 | 15 | <$5M | Research |
| Brix 27 LLC | Accommodation and Food Services | $171,300 | $172,994 | 31 | $5.5M | Research |
| Tri-State Surgical Center, LLC | Health Care and Social Assistance | $170,900 | $172,789 | 16 | $5.0M | Research |
| Martinsburg Institute, INC | Health Care and Social Assistance | $168,900 | $170,209 | 18 | <$5M | Research |
| Heritage Roofing, INC | Construction | $168,467 | $169,598 | 15 | <$5M | Research |