| Standard Laboratories INC | Professional, Scientific, and Technical Services | $3,667,400 | $3,540,016 | 352 | $71.0M |  Research |
| Clearon Corp. | Manufacturing | $3,341,600 | $2,876,468 | 124 | $123.0M | Research |
| Loved Ones in Home Care LLC | Health Care and Social Assistance | $1,831,000 | $1,851,650 | 495 | $84.8M | Research |
| Joe Holland Chevrolet, Inc. | Retail Trade | $1,537,800 | $1,448,471 | 139 | $35.0M | Research |
| Astorg Auto of Charleston, Inc. | Retail Trade | $1,400,000 | $1,410,694 | 91 | $22.9M | Research |
| Providence Enterprises, LLC | Retail Trade | $1,318,800 | $1,336,018 | 142 | $25.7M | Research |
| Psimed Corrections LLC | Health Care and Social Assistance | $1,256,300 | $1,263,287 | 99 | $23.2M | Research |
| Mid Atlantic Technology Research and Innovation Center INC | Professional, Scientific, and Technical Services | $1,221,500 | $1,231,741 | 78 | $16.2M | Research |
| Prime INC | Construction | $1,123,340 | $1,129,772 | 42 | $10.6M | Research |
| Prime, Inc. | Construction | $1,123,300 | $1,135,979 | 42 | $14.3M | Research |
| Pulmonary Associates of Charleston Pllc | Health Care and Social Assistance | $1,084,087 | $1,096,674 | 70 | $22.0M | Research |
| Psychological Assessment and Intervention Services, Inc. | Health Care and Social Assistance | $1,033,400 | $1,040,634 | 250 | $44.5M | Research |
| South Charleston Cardiology Associates Pllc | Health Care and Social Assistance | $756,677 | $269,399 | 22 | $6.9M | Research |
| City Bible Center | Other Services (except Public Administration) | $700,400 | $704,112 | 76 | N/A | Research |
| Capital Hotel Group LLC | Accommodation and Food Services | $641,276 | $649,559 | 68 | $21.3M | Research |
| Toms Plumbing INC | Construction | $636,900 | $644,101 | 52 | $16.5M | Research |
| Komax LLC | Real Estate and Rental and Leasing | $517,000 | $520,906 | 45 | $20.3M | Research |
| Komax LLC | Other Services (except Public Administration) | $517,000 | $524,396 | 45 | $9.7M | Research |
| South Charleston Electric Company | Construction | $479,097 | $481,985 | 30 | $9.9M | Research |
| Garlow Insurance Agency, Inc. | Finance and Insurance | $476,400 | $479,702 | 44 | $22.6M | Research |
| Office & Commercial Cleaning -Wv LLC | Administrative and Support and Waste Management | $464,600 | $467,168 | 103 | $22.3M | Research |
| Office & Commercial Cleaning-Wv, LLC | Administrative and Support and Waste Management | $442,000 | $445,278 | 103 | $22.3M | Research |
| Touchstone Testing Laboratory LLC | Other Services (except Public Administration) | $430,900 | $435,616 | 43 | $8.2M | Research |
| Raines Imports INC | Retail Trade | $353,100 | $357,782 | 33 | $8.3M | Research |
| Racocat Rubber, Inc. | Manufacturing | $331,500 | $321,797 | 27 | $26.8M | Research |