| West Virginia Regional Technology Park Corp | Real Estate and Rental and Leasing | $303,300 | $307,201 | 16 | N/A |  Research |
| Pauley Motor Car Co Preowned Vehicles LLC | Retail Trade | $300,600 | $303,079 | 26 | $6.6M | Research |
| L & L Arts LLC | Other Services (except Public Administration) | $298,524 | $300,127 | 50 | $9.6M | Research |
| South Charleston Electric Company, Inc. | Construction | $283,638 | $285,213 | 29 | $9.5M | Research |
| Charleston Acoustics INC | Wholesale Trade | $274,400 | $276,953 | 33 | $10.1M | Research |
| Raocat Rubber INC | Manufacturing | $273,506 | $274,817 | 23 | $22.8M | Research |
| Hospitality Ventures Limited Liability Company | Accommodation and Food Services | $268,804 | $272,209 | 30 | $9.4M | Research |
| Damous Psychological Services Pllc | Health Care and Social Assistance | $260,160 | $262,555 | 21 | <$5M | Research |
| Amcox Oil and Gas, LLC | Mining, Quarrying, and Oil and Gas Extraction | $258,000 | $260,170 | 16 | <$5M | Research |
| Calvin Broyles Jewelers LLC | Retail Trade | $242,900 | $245,842 | 21 | $5.8M | Research |
| Solutions Positive Behavior Strategies | Educational Services | $224,305 | $225,927 | 33 | <$5M | Research |
| West Virginia Employee Benefit Services INC | Finance and Insurance | $222,500 | $224,589 | 26 | $13.4M | Research |
| Charleston Nephrology | Health Care and Social Assistance | $222,200 | $199,206 | 25 | $7.8M | Research |
| Solutions Positive Behavioral Strategies LLC | Health Care and Social Assistance | $218,007 | $220,103 | 37 | $6.6M | Research |
| Associated Systems Professionals, LLC | Professional, Scientific, and Technical Services | $216,630 | $218,120 | 15 | <$5M | Research |
| Renal Consultants P L L C | Health Care and Social Assistance | $210,721 | $213,457 | 20 | $6.3M | Research |
| The Rheumatology Group, Pllc | Health Care and Social Assistance | $210,200 | $212,538 | 20 | <$5M | Research |
| Panucci & Jackfert Orthodontics INC | Health Care and Social Assistance | $193,000 | $194,445 | 22 | $5.1M | Research |
| Rocas INC | Accommodation and Food Services | $190,000 | $192,502 | 40 | $7.1M | Research |
| Harley-Davidson of West Virginia INC | Retail Trade | $189,710 | $190,880 | 18 | <$5M | Research |
| Harley-Davidson of West Virginia, Inc. | Retail Trade | $189,700 | $191,834 | 24 | $6.3M | Research |
| Be Enterprises, Inc. | Construction | $187,600 | $189,445 | 20 | $6.3M | Research |
| Be Enterprises Inc. | Construction | $187,600 | $190,247 | 20 | $6.3M | Research |
| Family Health Associates of the Kanawha | Health Care and Social Assistance | $186,800 | $188,398 | 19 | $6.0M | Research |
| Doan LLC | Accommodation and Food Services | $182,398 | $183,832 | 24 | <$5M | Research |