| Central Iowa Towing and Recovery, Inc. | Transportation and Warehousing | $309,602 | $312,182 | 41 | <$5M | AI Deep Dive |
| Technical Services Incorporated | Professional, Scientific, and Technical Services | $308,752 | $309,936 | 29 | <$5M | AI Deep Dive |
| Technical Services Incorporated | Professional, Scientific, and Technical Services | $308,700 | $310,679 | 29 | <$5M | AI Deep Dive |
| El Azteca, Inc. | Accommodation and Food Services | $308,100 | $310,675 | 52 | $9.2M | AI Deep Dive |
| Mb Enterprises of Ames, Lc | Construction | $307,915 | $311,382 | 25 | $7.9M | AI Deep Dive |
| St. Cecilia Church | Other Services (except Public Administration) | $307,652 | $309,296 | 36 | N/A | AI Deep Dive |
| Proplanner Inc. | Real Estate and Rental and Leasing | $300,000 | $301,667 | 20 | <$5M | AI Deep Dive |
| Ye Olde LLC | Accommodation and Food Services | $299,860 | $302,867 | 31 | $5.5M | AI Deep Dive |
| Ames Therapy Services | Health Care and Social Assistance | $299,605 | $301,263 | 17 | <$5M | AI Deep Dive |
| Sargent Metal Fabricating Inc. | Manufacturing | $299,600 | $301,223 | 25 | $6.1M | AI Deep Dive |
| Sargent Metal Fabricating INC | Manufacturing | $299,600 | $301,556 | 25 | $6.1M | AI Deep Dive |
| Cit Charters, INC | Transportation and Warehousing | $295,400 | $274,154 | 24 | $9.2M | AI Deep Dive |
| Cit Charters Inc. | Transportation and Warehousing | $295,400 | $297,659 | 24 | $9.2M | AI Deep Dive |
| Doran and Doran PC | Health Care and Social Assistance | $291,000 | $292,738 | 23 | $7.2M | AI Deep Dive |
| Mg Biologics, Inc. | Manufacturing | $283,100 | $285,593 | 35 | $34.7M | AI Deep Dive |
| Van Bergen & Associates, INC | Manufacturing | $281,707 | $283,150 | 21 | $5.9M | AI Deep Dive |
| Risk Assessment and Management, Inc. | Finance and Insurance | $276,634 | $278,494 | 13 | $6.7M | AI Deep Dive |
| Electronic Print, INC | Health Care and Social Assistance | $275,800 | $278,428 | 81 | $13.1M | AI Deep Dive |
| Risk Assessment and Management, Inc. | Finance and Insurance | $274,169 | $275,326 | 15 | $7.7M | AI Deep Dive |
| Hunziker Construction Services | Construction | $260,210 | $261,928 | 14 | <$5M | AI Deep Dive |
| Aunt Maude's of Ames LLC | Accommodation and Food Services | $257,300 | $259,492 | 24 | <$5M | AI Deep Dive |
| Brown's Heavy Equipment, Inc. | Other Services (except Public Administration) | $254,718 | $255,457 | 18 | <$5M | AI Deep Dive |
| Christ Community Church | Other Services (except Public Administration) | $254,640 | $257,102 | 23 | N/A | AI Deep Dive |
| Haila Architecture Structure Planning, Ltd. | Professional, Scientific, and Technical Services | $253,800 | $255,205 | 13 | <$5M | AI Deep Dive |
| Clyde's of Ames, Inc. | Accommodation and Food Services | $252,300 | $254,229 | 35 | $6.2M | AI Deep Dive |