| Mechanical Comfort, Inc. | Construction | $203,172 | $205,277 | 24 | $7.6M |  AI Deep Dive |
| Cyclone Holdings, LLC | Administrative and Support and Waste Management | $200,800 | $201,971 | 34 | $6.4M | AI Deep Dive |
| Mti Biotech Incorporated | Manufacturing | $199,000 | $200,319 | 14 | <$5M | AI Deep Dive |
| Pizza Ranch of Ames LLC | Accommodation and Food Services | $198,468 | $199,648 | 39 | $6.9M | AI Deep Dive |
| Heemco, Inc. | Construction | $197,500 | $199,161 | 16 | <$5M | AI Deep Dive |
| Kta LLC | Finance and Insurance | $193,750 | $194,832 | 11 | $5.7M | AI Deep Dive |
| Veterinary Hospital of Somerset PC | Professional, Scientific, and Technical Services | $193,424 | $195,435 | 18 | <$5M | AI Deep Dive |
| Ames Convention & Visitors Bureau | Administrative and Support and Waste Management | $192,482 | $194,485 | 16 | $5.1M | AI Deep Dive |
| Gateway Expresse Inc. | Accommodation and Food Services | $192,108 | $193,170 | 26 | <$5M | AI Deep Dive |
| Ames Engineering, Inc. | Professional, Scientific, and Technical Services | $191,957 | $193,414 | 12 | <$5M | AI Deep Dive |
| Keith Cooper and Sons Inc. | Construction | $191,850 | $193,055 | 17 | $6.4M | AI Deep Dive |
| Aunt Maude's of Ames, LLC | Accommodation and Food Services | $191,100 | $193,105 | 38 | $6.7M | AI Deep Dive |
| G. Wandling & Associates Inc. | Professional, Scientific, and Technical Services | $190,600 | $191,728 | 8 | <$5M | AI Deep Dive |
| Gary Thompson Oil Co. | Wholesale Trade | $189,615 | $190,205 | 69 | $17.8M | AI Deep Dive |
| Gary Thompson Oil Co. | Wholesale Trade | $189,615 | $191,327 | 33 | $8.5M | AI Deep Dive |
| Championship Productions, Inc. | Information | $186,409 | $187,439 | 16 | <$5M | AI Deep Dive |
| Ames Oral Surgeons PC | Health Care and Social Assistance | $186,250 | $187,250 | 18 | <$5M | AI Deep Dive |
| Ht Plumbing INC | Construction | $186,125 | $187,211 | 19 | $6.0M | AI Deep Dive |
| Ht Plumbing, Inc. | Construction | $186,125 | $187,640 | 15 | <$5M | AI Deep Dive |
| Dentistry at Somerset P.c. | Health Care and Social Assistance | $183,000 | $184,294 | 21 | <$5M | AI Deep Dive |
| Dentistry at Somerset, P.c. | Health Care and Social Assistance | $183,000 | $184,354 | 16 | <$5M | AI Deep Dive |
| Assault Care Center Extending Shelter & Support | Health Care and Social Assistance | $182,585 | $184,426 | 22 | <$5M | AI Deep Dive |
| Iso-Form LLC | Retail Trade | $182,300 | $180,183 | 13 | <$5M | AI Deep Dive |
| Newbrough Law Firm, LLP | Professional, Scientific, and Technical Services | $181,400 | $182,722 | 8 | <$5M | AI Deep Dive |
| Cyclone Contracting Corporation | Construction | $180,832 | $183,138 | 12 | <$5M | AI Deep Dive |