| Hokel Machine Supply, Inc. | Retail Trade | $151,700 | $152,535 | 19 | <$5M | AI Deep Dive |
| Iowa State Daily Publication Board, Inc. | Information | $150,572 | $151,475 | 11 | N/A | AI Deep Dive |
| Ames Heating & Cooling, Inc. | Construction | $150,000 | $151,064 | 15 | <$5M | AI Deep Dive |
| Db Iowa Holdings INC | Information | $150,000 | $150,458 | 17 | <$5M | AI Deep Dive |
| Zylstra Cycle Company INC | Retail Trade | $148,702 | $149,933 | 19 | <$5M | AI Deep Dive |
| Hgik Hospitality LLC | Accommodation and Food Services | $145,561 | $146,050 | 20 | $6.3M | AI Deep Dive |
| Gross-Wen Technologies INC | Administrative and Support and Waste Management | $143,900 | $144,704 | 15 | <$5M | AI Deep Dive |
| Tricke's LLC | Other Services (except Public Administration) | $142,500 | $143,542 | 9 | <$5M | AI Deep Dive |
| University Community Childcare | Health Care and Social Assistance | $142,313 | $143,380 | 46 | N/A | AI Deep Dive |
| Ames Silversmithing, Inc. | Retail Trade | $142,300 | $143,115 | 9 | <$5M | AI Deep Dive |
| Ingios Geotechnics INC | Professional, Scientific, and Technical Services | $141,940 | $142,582 | 12 | <$5M | AI Deep Dive |
| The Furman Corporation | Real Estate and Rental and Leasing | $141,304 | $142,407 | 10 | <$5M | AI Deep Dive |
| Brandon M. Patten, Dds, PC | Health Care and Social Assistance | $140,389 | $141,785 | 17 | <$5M | AI Deep Dive |
| Brandon M. Patten DDS PC | Health Care and Social Assistance | $140,389 | $140,935 | 16 | <$5M | AI Deep Dive |
| Famc | Accommodation and Food Services | $140,200 | $141,777 | 32 | $5.7M | AI Deep Dive |
| Flummerfelt Enterprises, Inc. | Real Estate and Rental and Leasing | $140,200 | $141,037 | 12 | <$5M | AI Deep Dive |
| Terry and Andy's Tacos, Inc. | Accommodation and Food Services | $140,116 | $141,191 | 11 | <$5M | AI Deep Dive |
| Zylstra Cycle Company Inc. | Retail Trade | $137,678 | $138,928 | 16 | <$5M | AI Deep Dive |
| Gateway Expresse Inc. | Real Estate and Rental and Leasing | $137,220 | $138,131 | 19 | <$5M | AI Deep Dive |
| Fisher Management, Inc. | Accommodation and Food Services | $137,100 | $138,113 | 28 | <$5M | AI Deep Dive |
| Ames Hotel Investment LLC | Accommodation and Food Services | $136,535 | $137,646 | 31 | $9.7M | AI Deep Dive |
| Terry and Andy's Tacos, Inc. | Accommodation and Food Services | $136,125 | $137,792 | 6 | <$5M | AI Deep Dive |
| Pyfferoen Pediatric Dentistry Pllc | Health Care and Social Assistance | $136,072 | $136,903 | 11 | <$5M | AI Deep Dive |
| Venuworks of Chesapeake LLC | Arts, Entertainment, and Recreation | $135,846 | $136,593 | 23 | $6.0M | AI Deep Dive |
| Ames Golf & Country Club | Arts, Entertainment, and Recreation | $135,570 | $127,508 | 20 | N/A | AI Deep Dive |