| Draintech, Inc. | Construction | $180,800 | $182,851 | 16 | $5.1M | AI Deep Dive |
| Championship Productions Inc. | Retail Trade | $180,632 | $181,475 | 15 | <$5M | AI Deep Dive |
| Iowa Subway Development INC | Professional, Scientific, and Technical Services | $178,927 | $180,259 | 9 | <$5M | AI Deep Dive |
| Ames Hotel Management Inc. | Accommodation and Food Services | $176,043 | $177,808 | 15 | <$5M | AI Deep Dive |
| Iowa State Daily Publication Board Inc. | Information | $175,308 | $175,849 | 11 | N/A | AI Deep Dive |
| New Stream II Lc | Unknown | $172,640 | $174,496 | 58 | N/A | AI Deep Dive |
| Ames Trenching and Excavating INC | Construction | $172,500 | $173,478 | 19 | $7.1M | AI Deep Dive |
| Fisher Management INC | Accommodation and Food Services | $172,082 | $173,320 | 28 | <$5M | AI Deep Dive |
| Gianello Inc. | Accommodation and Food Services | $168,865 | $169,822 | 17 | <$5M | AI Deep Dive |
| Jkm Midway | Accommodation and Food Services | $166,894 | $169,112 | 28 | <$5M | AI Deep Dive |
| Bad Company Inc. | Accommodation and Food Services | $165,505 | $167,684 | 39 | $6.9M | AI Deep Dive |
| Heuss Printing, INC | Manufacturing | $163,320 | $164,752 | 18 | $17.9M | AI Deep Dive |
| Bw Ames Investment LLC | Accommodation and Food Services | $162,088 | $163,502 | 11 | <$5M | AI Deep Dive |
| Real Estate Service Group, Inc. | Real Estate and Rental and Leasing | $162,023 | $163,266 | 13 | <$5M | AI Deep Dive |
| Venuworks of Brookings LLC | Arts, Entertainment, and Recreation | $161,165 | $0 | 25 | $6.6M | AI Deep Dive |
| Popp Engineering Inc. | Professional, Scientific, and Technical Services | $161,040 | $73,558 | 8 | <$5M | AI Deep Dive |
| Heuss Printing INC | Manufacturing | $159,817 | $161,336 | 14 | $13.9M | AI Deep Dive |
| Don and Sons Body Shop, Inc. | Other Services (except Public Administration) | $159,797 | $160,660 | 11 | <$5M | AI Deep Dive |
| Dan's Custom Landscapes, INC | Administrative and Support and Waste Management | $159,280 | $160,568 | 26 | $5.6M | AI Deep Dive |
| First Property Management Ames, Lc | Professional, Scientific, and Technical Services | $159,237 | $138,738 | 15 | <$5M | AI Deep Dive |
| Aspi Solutions Inc. | Information | $158,084 | $159,162 | 10 | <$5M | AI Deep Dive |
| Don and Sons Body Shop, Inc. | Other Services (except Public Administration) | $155,600 | $156,559 | 10 | <$5M | AI Deep Dive |
| The Bernard Firm Plc | Professional, Scientific, and Technical Services | $155,264 | $156,294 | 23 | $5.6M | AI Deep Dive |
| J.a. Smith Machinery Co., Ltd | Manufacturing | $154,800 | $155,664 | 14 | <$5M | AI Deep Dive |
| Pizza Ranch of Ames LLC | Accommodation and Food Services | $152,300 | $153,675 | 39 | $6.9M | AI Deep Dive |