| Hunziker & Associates Inc, Realtors | Real Estate and Rental and Leasing | $242,957 | $244,561 | 20 | <$5M | AI Deep Dive |
| Waterpro Supplies, Inc. | Wholesale Trade | $242,937 | $244,314 | 20 | $6.1M | AI Deep Dive |
| New Stream II Lc | Accommodation and Food Services | $241,692 | $244,492 | 41 | $7.3M | AI Deep Dive |
| Global Reach Internet Productions, LLC | Professional, Scientific, and Technical Services | $240,025 | $242,385 | 20 | <$5M | AI Deep Dive |
| Beth & Kelly, Inc. | Management of Companies and Enterprises | $237,825 | $239,356 | 16 | <$5M | AI Deep Dive |
| Ames Community Preschool Center | Educational Services | $236,570 | $238,113 | 40 | N/A | AI Deep Dive |
| Clear Brook Counseling Professionals, LLC | Health Care and Social Assistance | $235,674 | $237,658 | 14 | <$5M | AI Deep Dive |
| Ames Community Preschool Center | Educational Services | $235,322 | $236,399 | 62 | N/A | AI Deep Dive |
| Jensen Golf L.c. | Arts, Entertainment, and Recreation | $234,000 | $235,547 | 19 | <$5M | AI Deep Dive |
| Bad Company Inc. | Accommodation and Food Services | $231,709 | $233,505 | 39 | $6.9M | AI Deep Dive |
| Jerry Carney and Sons INC | Retail Trade | $231,700 | $209,565 | 24 | $6.5M | AI Deep Dive |
| Thirty View Inc. | Construction | $230,395 | $231,752 | 22 | $7.3M | AI Deep Dive |
| Thirty View, Inc. | Construction | $226,800 | $228,583 | 19 | $6.3M | AI Deep Dive |
| Behle, Inc. | Administrative and Support and Waste Management | $226,775 | $228,759 | 19 | <$5M | AI Deep Dive |
| Door and Fence Store, Inc. | Construction | $223,600 | $226,461 | 29 | $9.3M | AI Deep Dive |
| Kurrent Electric, Inc. | Construction | $220,867 | $222,143 | 33 | $10.9M | AI Deep Dive |
| Keith Cooper and Sons, Inc. | Construction | $220,000 | $221,839 | 19 | $5.9M | AI Deep Dive |
| Ye Olde, L.l.c. | Accommodation and Food Services | $216,600 | $218,256 | 30 | $5.3M | AI Deep Dive |
| J.a. Smith Machinery Company Ltd | Manufacturing | $215,600 | $216,187 | 26 | $6.8M | AI Deep Dive |
| Electric Wholesale Company | Wholesale Trade | $213,000 | $214,179 | 20 | $6.1M | AI Deep Dive |
| Conley's Trucking, Inc. | Transportation and Warehousing | $212,800 | $214,355 | 18 | <$5M | AI Deep Dive |
| Ames Chamber of Commerce | Other Services (except Public Administration) | $212,536 | $214,358 | 29 | $5.5M | AI Deep Dive |
| Jerry Carney and Sons Inc. | Wholesale Trade | $211,205 | $212,767 | 24 | $7.4M | AI Deep Dive |
| Ames Oral Surgeons P.c | Health Care and Social Assistance | $208,520 | $209,748 | 17 | <$5M | AI Deep Dive |
| Iowa Subway Development, Inc. | Accommodation and Food Services | $204,500 | $206,483 | 14 | <$5M | AI Deep Dive |