| Molo Oil Company | Wholesale Trade | $655,700 | $657,977 | 226 | $58.2M | AI Deep Dive |
| Great River Oral and Maxillofacial Surgery, P.c. | Health Care and Social Assistance | $642,400 | $647,275 | 53 | $12.3M | AI Deep Dive |
| Roeder Implement Inc. | Wholesale Trade | $640,943 | $646,369 | 52 | $15.9M | AI Deep Dive |
| Twi of Dubuque Inc. | Other Services (except Public Administration) | $640,900 | $644,318 | 219 | $41.8M | AI Deep Dive |
| Roeder Implement, Incorporated | Wholesale Trade | $640,900 | $645,834 | 53 | $16.2M | AI Deep Dive |
| Mike Finnin Ford L.l.c | Retail Trade | $628,700 | $634,677 | 66 | $16.6M | AI Deep Dive |
| Great River Oral and Maxillofacial Surgery P.c. | Health Care and Social Assistance | $623,693 | $627,059 | 51 | $11.8M | AI Deep Dive |
| Platinum Holdings LLC | Accommodation and Food Services | $616,198 | $621,516 | 108 | $33.8M | AI Deep Dive |
| Geisler Brothers Co. | Construction | $605,300 | $610,395 | 60 | $19.0M | AI Deep Dive |
| Jim Giese Commercial Roofing Inc. | Construction | $604,400 | $607,556 | 39 | $12.9M | AI Deep Dive |
| Mulgrew Oil Co. | Wholesale Trade | $600,000 | $606,115 | 36 | $9.3M | AI Deep Dive |
| Hjd Subtenant LLC | Accommodation and Food Services | $596,700 | $602,402 | 154 | $48.2M | AI Deep Dive |
| Domino's Pizza | Accommodation and Food Services | $594,300 | $600,094 | 212 | $37.6M | AI Deep Dive |
| Fiegen & Bisenius INC | Educational Services | $591,500 | $595,164 | 80 | $16.8M | AI Deep Dive |
| Pbs Financial Systems INC | Professional, Scientific, and Technical Services | $582,600 | $585,776 | 45 | $9.3M | AI Deep Dive |
| Mulgrew Oil Co. | Wholesale Trade | $581,788 | $587,574 | 36 | $9.3M | AI Deep Dive |
| Ennoble Nursing LLC | Health Care and Social Assistance | $576,880 | $581,912 | 87 | $14.8M | AI Deep Dive |
| Dubuque County Historical Society | Arts, Entertainment, and Recreation | $566,200 | $568,764 | 135 | $20.5M | AI Deep Dive |
| Dubuque County Historical Society | Arts, Entertainment, and Recreation | $566,200 | $570,023 | 62 | $9.4M | AI Deep Dive |
| O'connor & Thomas, P.c. | Professional, Scientific, and Technical Services | $565,000 | $570,007 | 34 | $8.3M | AI Deep Dive |
| Green Industrial Supply, Inc. | Transportation and Warehousing | $562,100 | $568,999 | 51 | $12.9M | AI Deep Dive |
| Comelec Services, Inc. | Information | $541,800 | $546,209 | 46 | $7.7M | AI Deep Dive |
| Deery Brothers of Dubuque, Inc. | Retail Trade | $536,900 | $539,901 | 37 | $9.3M | AI Deep Dive |
| Green Industrial Supply Inc. | Transportation and Warehousing | $534,342 | $539,188 | 52 | $13.2M | AI Deep Dive |
| Dubuque Anesthesia Services PC | Health Care and Social Assistance | $528,812 | $532,188 | 20 | $6.3M | AI Deep Dive |