| Guardian Healthcare | Health Care and Social Assistance | $528,162 | $532,945 | 83 | $14.1M |  AI Deep Dive |
| Double Dragon International | Wholesale Trade | $527,900 | $532,021 | 37 | $11.3M | AI Deep Dive |
| Tri-Technical Systems, Inc. | Professional, Scientific, and Technical Services | $523,700 | $529,708 | 43 | $8.9M | AI Deep Dive |
| Fiegen & Bisenius Inc. | Educational Services | $523,500 | $527,121 | 88 | $18.5M | AI Deep Dive |
| Fiber Chem, Inc. | Construction | $512,600 | $516,757 | 38 | $9.6M | AI Deep Dive |
| Fiber Chem Inc. | Construction | $512,600 | $515,212 | 33 | $10.2M | AI Deep Dive |
| Dittmer Recycling, Inc. | Administrative and Support and Waste Management | $501,500 | $505,265 | 46 | $8.7M | AI Deep Dive |
| Deery Brothers of Dubuque Inc. | Retail Trade | $501,400 | $503,103 | 36 | $9.1M | AI Deep Dive |
| Turf Management, LLC | Administrative and Support and Waste Management | $498,400 | $504,547 | 65 | $14.1M | AI Deep Dive |
| Mde LLC | Construction | $494,505 | $496,744 | 22 | $8.2M | AI Deep Dive |
| Mde, LLC DBA Mcdermott Excavating | Construction | $494,500 | $498,319 | 25 | $9.4M | AI Deep Dive |
| The Floor Show Corporation | Retail Trade | $483,454 | $486,037 | 46 | $8.3M | AI Deep Dive |
| The Floor Show Corporation | Retail Trade | $483,400 | $487,996 | 43 | $7.8M | AI Deep Dive |
| Dubuque Hotel Partners | Accommodation and Food Services | $475,702 | $479,389 | 75 | $23.5M | AI Deep Dive |
| Grobstick Electrical, Inc. | Construction | $462,900 | $468,569 | 43 | $14.1M | AI Deep Dive |
| Harris Motor Sports, Inc. | Retail Trade | $444,000 | $449,389 | 46 | $12.0M | AI Deep Dive |
| Paramount Emergency Medical Service INC | Health Care and Social Assistance | $440,000 | $442,713 | 42 | $9.7M | AI Deep Dive |
| Tc Networks, INC | Professional, Scientific, and Technical Services | $434,410 | $438,513 | 33 | $6.8M | AI Deep Dive |
| Tc Networks INC | Professional, Scientific, and Technical Services | $434,409 | $436,774 | 33 | $6.8M | AI Deep Dive |
| Paramount Emergency Medical Service INC | Health Care and Social Assistance | $433,400 | $436,807 | 56 | $13.0M | AI Deep Dive |
| Edwards Cast Stone Co. | Construction | $429,076 | $411,287 | 40 | $13.6M | AI Deep Dive |
| F. H. Uelner Precision Tools & Dies Inc. | Manufacturing | $428,081 | $430,380 | 37 | $9.7M | AI Deep Dive |
| Dubuque Orthopaedic Surgeons | Health Care and Social Assistance | $426,800 | $310,342 | 22 | $5.1M | AI Deep Dive |
| Great River Professional Services, Inc. | Administrative and Support and Waste Management | $424,500 | $429,405 | 32 | $5.0M | AI Deep Dive |
| Eagle Point Software Corporation | Information | $418,300 | $422,948 | 22 | <$5M | AI Deep Dive |