| Dubuque Obstetrics and Gynecology | Health Care and Social Assistance | $333,300 | $335,179 | 25 | <$5M | AI Deep Dive |
| Mccullough Creative Inc. | Professional, Scientific, and Technical Services | $331,410 | $352,353 | 25 | <$5M | AI Deep Dive |
| Giese Roofing Company | Construction | $331,000 | $333,685 | 24 | $7.9M | AI Deep Dive |
| Unified Therapy Services INC | Health Care and Social Assistance | $328,964 | $331,715 | 41 | $11.1M | AI Deep Dive |
| Dairy Lab Services, Inc. | Professional, Scientific, and Technical Services | $326,900 | $329,043 | 40 | N/A | AI Deep Dive |
| Tri-State Industries INC | Manufacturing | $323,628 | $327,331 | 31 | $8.1M | AI Deep Dive |
| Union-Hoermann Press, Inc. | Manufacturing | $321,800 | $323,641 | 22 | $21.8M | AI Deep Dive |
| Union-Hoermann Press, Inc. | Manufacturing | $318,055 | $319,548 | 21 | $20.8M | AI Deep Dive |
| Steve's Ace Hardware, Inc. | Wholesale Trade | $313,900 | $317,196 | 39 | $11.9M | AI Deep Dive |
| Steele Capital Management INC | Finance and Insurance | $313,800 | $317,505 | 12 | $6.2M | AI Deep Dive |
| Unified Therapy Services, INC | Health Care and Social Assistance | $309,500 | $312,380 | 34 | $9.2M | AI Deep Dive |
| Dubuque Steel Products INC | Manufacturing | $308,000 | $311,063 | 51 | $12.5M | AI Deep Dive |
| Giese Roofing Company | Construction | $304,300 | $306,650 | 21 | <$5M | AI Deep Dive |
| Mcgrath Motorcycles of Dubuque LLC | Retail Trade | $298,000 | $300,127 | 20 | $5.2M | AI Deep Dive |
| Mcdevitt Dental, P.c. | Health Care and Social Assistance | $296,052 | $297,861 | 21 | <$5M | AI Deep Dive |
| Dubuque Supply Co. | Wholesale Trade | $292,800 | $294,998 | 28 | $8.6M | AI Deep Dive |
| Langworthy Dental Group PC | Health Care and Social Assistance | $291,500 | $294,569 | 20 | <$5M | AI Deep Dive |
| P & K Enterprises, Inc. | Accommodation and Food Services | $289,700 | $292,668 | 105 | $18.6M | AI Deep Dive |
| Gronen Restoration INC | Construction | $288,493 | $290,983 | 36 | $10.9M | AI Deep Dive |
| Langworthy Dental Group PC | Health Care and Social Assistance | $288,300 | $290,430 | 24 | $5.6M | AI Deep Dive |
| Community Foundation of Greater Dubuque | Finance and Insurance | $284,200 | $285,889 | 25 | N/A | AI Deep Dive |
| Careerpros LLC | Administrative and Support and Waste Management | $283,289 | $286,060 | 23 | <$5M | AI Deep Dive |
| Careerpros, LLC | Administrative and Support and Waste Management | $283,200 | $286,117 | 26 | <$5M | AI Deep Dive |
| Fuerste Eye Clinic, P.c. | Health Care and Social Assistance | $280,700 | $283,780 | 26 | $6.1M | AI Deep Dive |
| Sunflower Enterprises Inc. | Construction | $280,633 | $282,248 | 14 | <$5M | AI Deep Dive |