| Agri Power, INC | Wholesale Trade | $6,214,819 | $6,287,843 | 450 | $137.9M |  Research |
| Lee Masonry Products, Inc. | Construction | $2,904,800 | $2,937,963 | 350 | $81.0M | Research |
| Electronic Metrology Laboratory, LLC | Professional, Scientific, and Technical Services | $2,520,102 | $156,885 | 237 | $47.8M | Research |
| Pennyrile Rural Electric Cooperative Corporation | Utilities | $1,987,500 | $2,008,755 | 111 | N/A | Research |
| Pennyrile Allied Community Services INC | Health Care and Social Assistance | $1,425,617 | $1,437,299 | 267 | N/A | Research |
| Max S. Arnnold & Sons, LLC | Wholesale Trade | $1,356,532 | $1,371,303 | 260 | $66.9M | Research |
| Max Arnold & Sons LLC | Wholesale Trade | $1,216,625 | $1,222,978 | 213 | $54.8M | Research |
| General Steel Contractors INC | Construction | $1,001,600 | $865,400 | 41 | $12.7M | Research |
| Comefri USA INC | Manufacturing | $973,472 | $978,556 | 91 | $23.8M | Research |
| Sisk Motor Company | Retail Trade | $890,375 | $897,078 | 69 | $17.4M | Research |
| Cayce Mill Supply, Inc. | Wholesale Trade | $879,200 | $884,255 | 85 | $26.0M | Research |
| Mid-Continent Spring Co | Manufacturing | $863,482 | $868,615 | 95 | $23.2M | Research |
| Hopkinsville Elevator Co., INC | Wholesale Trade | $828,582 | $833,185 | 58 | $14.9M | Research |
| Mid-Continent Spring Company | Manufacturing | $789,727 | $794,158 | 89 | $21.8M | Research |
| Flynn Enterprises LLC | Manufacturing | $774,682 | $778,749 | 60 | $21.2M | Research |
| Agri-Chem LLC | Manufacturing | $768,027 | $773,041 | 49 | $48.6M | Research |
| Hopkinsville Elevator Co INC | Wholesale Trade | $757,371 | $761,705 | 60 | $15.4M | Research |
| Cornerstone Information Systems Inc. | Professional, Scientific, and Technical Services | $728,000 | $734,572 | 49 | $10.1M | Research |
| Cornerstone Information Systems, Inc. | Professional, Scientific, and Technical Services | $728,000 | $734,188 | 45 | $9.3M | Research |
| Watkins Express Freight Inc. | Transportation and Warehousing | $659,993 | $668,335 | 80 | $21.4M | Research |
| Watkins Express Freight INC | Transportation and Warehousing | $648,363 | $653,946 | 94 | $18.9M | Research |
| Commonwealth Agri-Energy, LLC | Manufacturing | $553,412 | $556,533 | 34 | $33.7M | Research |
| Renshaw, Inc. | Retail Trade | $529,000 | $534,026 | 50 | $12.6M | Research |
| Western Kentucky Pulmonary Clinic | Health Care and Social Assistance | $520,152 | $523,663 | 60 | $11.9M | Research |
| Protank and Equipment LLC | Wholesale Trade | $500,382 | $503,815 | 51 | $15.6M | Research |