| Dunlap Sunbrand International, Inc. | Wholesale Trade | $250,237 | $251,808 | 22 | $6.7M |  Research |
| Dunlap Sunbrand International INC | Wholesale Trade | $250,237 | $252,114 | 22 | $6.7M | Research |
| Victory Praise LLC | Manufacturing | $246,635 | $248,320 | 39 | $10.2M | Research |
| Southern States Hopkinsville | Transportation and Warehousing | $237,500 | $238,833 | 18 | <$5M | Research |
| Porta Carport INC | Manufacturing | $232,000 | $234,127 | 23 | $5.6M | Research |
| Southern States Hopkinsville Petroleum Cooperative INC | Transportation and Warehousing | $227,400 | $228,752 | 13 | <$5M | Research |
| Southern States Hopkinsville Petroleum Cooperative INC | Transportation and Warehousing | $227,400 | $228,891 | 12 | <$5M | Research |
| Southern States Hopkinsville Cooperative INC | Transportation and Warehousing | $224,300 | $225,876 | 22 | $5.6M | Research |
| Calvert Ophthalmology Center Psc | Health Care and Social Assistance | $221,200 | $223,836 | 26 | $6.1M | Research |
| Kentucky Western Truck Lines INC | Transportation and Warehousing | $220,885 | $222,124 | 23 | <$5M | Research |
| Sts. Peter & Paul Parish of Hopkinsville, Kentucky | Other Services (except Public Administration) | $215,217 | $216,691 | 24 | N/A | Research |
| Skyline Animal Clinic Psc | Health Care and Social Assistance | $213,300 | $214,716 | 28 | $8.8M | Research |
| Generations Primary Care, Psc | Health Care and Social Assistance | $212,317 | $213,473 | 16 | <$5M | Research |
| Generations Primary Care Psc | Health Care and Social Assistance | $212,317 | $213,461 | 16 | $5.0M | Research |
| Pennyrile Home Medical | Wholesale Trade | $206,050 | $207,458 | 16 | <$5M | Research |
| Empire Hotels INC | Accommodation and Food Services | $202,500 | $205,172 | 18 | $5.6M | Research |
| Renshaw & Renshaw Management Group | Administrative and Support and Waste Management | $201,751 | $201,378 | 8 | <$5M | Research |
| Richgrove Tobacco, LLC | Agriculture, Forestry, Fishing and Hunting | $197,200 | $198,436 | 73 | $7.5M | Research |
| Knight Masonry & Sons LLC | Construction | $195,341 | $196,984 | 17 | <$5M | Research |
| City Salvage & Recycling INC | Wholesale Trade | $193,800 | $194,995 | 24 | $7.4M | Research |
| City Salvage & Recycling INC | Wholesale Trade | $193,800 | $195,124 | 23 | $7.0M | Research |
| Pennyrile Radiology, Psc | Health Care and Social Assistance | $193,107 | $194,384 | 14 | <$5M | Research |
| Marsh Construction Company, Inc. | Construction | $190,460 | $191,523 | 22 | $25.1M | Research |
| Family Restaurant Group INC | Accommodation and Food Services | $186,848 | $187,969 | 25 | <$5M | Research |
| Hopkinsville-Christian County Family Ymca INC | Arts, Entertainment, and Recreation | $186,300 | $188,241 | 75 | $10.5M | Research |